Anti-CD158a/KIR2DL1 Monoclonal Antibody for Flow Cytometry - P43626

Core Product Specifications and Parameters

Biological Background: CD158a/KIR2DL1 Function and Localization

• CD158a/KIR2DL1 (Killer cell immunoglobulin-like receptor 2DL1) is also known as NKAT1, p58.1, CD158A, and several other aliases, reflecting its history as an NK cell-associated transcript and MHC class I receptor.
• It functions as an inhibitory receptor on natural killer (NK) cells, recognizing specific HLA-C alleles (e.g., w4 and w6) and transmitting signals that prevent target cell lysis.
• The protein belongs to the killer cell immunoglobulin-like receptor (KIR) family, characterized by multiple extracellular immunoglobulin domains; alternative splicing generates multiple isoforms.
• Subcellularly, KIR2DL1 is a single-pass transmembrane protein located on the cell membrane, with its ligand-binding domains exposed extracellularly.
• Post-translational modifications include glycosylation and disulfide bond formation, contributing to the observed molecular weight of 38-41 kDa, compared to the predicted 38.6 kDa.
• It is specifically expressed by NK cells and some T-cell subsets, playing a crucial role in immune surveillance and self-tolerance.
• Structurally resolved in 3D, its specificity for HLA-C is determined by polymorphisms in the extracellular domain, which are key to its inhibitory function.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 22-245) encompasses the entire extracellular region, making the antibody well-suited for detecting native surface receptor by flow cytometry.
• Optimize staining by titrating the antibody on relevant human NK cell lines (e.g., NKL) or primary NK cells to determine the optimal concentration for specific binding.
• Consider co-staining with anti-CD56 and anti-CD3 to precisely gate on NK cells (CD3-CD56+) and distinguish KIR2DL1-positive subsets.
• For functional studies, validate the antibody in the presence of target cells expressing cognate HLA-C ligands to confirm inhibitory signaling blockade.
• Note that cross-reactivity is limited to human; performance in other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material provider, CamelBio streamlines access to high-quality antibodies, recombinant proteins, and ancillary reagents for diagnostic and research applications. For studies in NK cell biology and immuno-oncology, we offer validated anti-KIR2DL1 monoclonal antibodies and can facilitate custom development, bulk supply, and technical consulting to accelerate your projects from concept to clinic.