Anti-CD157/BST-1 Monoclonal Antibody for Flow Cytometry - Q10588

Core Product Specifications and Parameters

Biological Background: CD157/BST-1 Function and Localization

• CD157/BST-1 is an ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 that catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD(+) and its hydrolysis to ADP-ribose (ADPR) (PubMed:7805847). Related references: PMID:7805847
• It functions as a GPI-anchored glycoprotein on the cell membrane, acting as an ectoenzyme.
• cADPR serves as a second messenger that mobilizes intracellular calcium stores, potentially regulating calcium-dependent processes.
• The protein is expressed in various tissues including placenta, lung, liver, and kidney, suggesting broad physiological roles.
• Alternative splicing generates at least two isoforms, with reported molecular weights of approximately 29-36 kDa.
• May be involved in pre-B-cell growth, indicating a role in immune cell development.
• Contains structural features such as disulfide bonds, alternative splicing, and GPI-anchor.
• Also known as bone marrow stromal cell antigen 1 (BST-1), it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen targets the extracellular domain (amino acids 29-292) of human CD157/BST-1, suggesting the antibody may recognize the native protein on live cells.
• As a directly conjugated primary antibody (), it eliminates the need for secondary staining and is optimized for flow cytometry.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• For surface staining, standard protocols can be applied; intracellular staining requires appropriate fixation and permeabilization.
• Validate the antibody in human cell lines or primary cells known to express CD157/BST-1 (e.g., bone marrow stromal cells, placental cells) to confirm reactivity.
• The cross-reactivity is specified for human; performance in other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including monoclonal antibodies, recombinant proteins, and ancillary reagents, supporting your diagnostic development from concept to clinic. This anti-CD157/BST-1 product exemplifies our commitment to enabling research in cyclic ADP-ribose signaling and immune cell biology. Contact us for customized sourcing solutions, bulk supplies, or assistance with rare-target reagent procurement.