Anti-CD155/PVR Rabbit Monoclonal Antibody for WB, ELISA - P15151

Core Product Specifications and Parameters

Biological Background: CD155/PVR Function and Localization

• CD155 (poliovirus receptor, PVR) is a member of the nectin family of immunoglobulin-like adhesion molecules, also known as nectin-like protein 5 (NECL-5). The PVR gene undergoes alternative splicing to generate multiple isoforms.
• It functions as a critical NK cell adhesion molecule by binding CD96 and CD226. These interactions accumulate at the cell-cell contact site, promoting the formation of a mature immunological synapse that triggers NK cell cytotoxicity and IFN-gamma secretion.
• CD155 plays a role in tumor cell invasion and migration. On tumor cells, high PVR expression can lead to fratricide NK cell activation, providing a mechanism of immune evasion.
• As a host cell receptor for virus entry, CD155 mediates poliovirus infection by directing virion-PVR-containing endocytic vesicles to the microtubular network via interaction with DYNLT1, facilitating axonal retrograde transport. It also acts as a receptor for Pseudorabies virus.
• Human cytomegalovirus (HHV-5) can prevent CD155 from reaching the cell surface, likely to escape immune recognition of infected cells by NK cells.
• The 45 kDa transmembrane glycoprotein contains Ig-like domains and features disulfide bonds, phosphorylation sites, and a signal peptide. It localizes to the cell membrane and is also secreted, consistent with its roles in adhesion and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 250–350 of CD155, which lies in the extracellular domain. This design may make the antibody suitable for detecting the extracellular portion in applications like ELISA.
• For Western blot, the predicted molecular weight is approximately 45 kDa. Consider using a suitable gel percentage (e.g., 10% SDS-PAGE) and standard wet or semi-dry transfer conditions for proteins of this size.
• As cross-reactivity is confirmed for human, validate the antibody in relevant human samples such as NK cells, tumor cell lines, or tissues expressing CD155.
• In ELISA, the rabbit monoclonal antibody can be explored as a capture or detection component due to its high specificity. Titration and pairing experiments are recommended to optimize assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with seamless one-stop access to high-quality IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. For CD155/PVR-focused studies in immuno-oncology, NK cell biology, or virology, we offer this optimized rabbit monoclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to efficiently secure the biological reagents you need.