Anti-CD155/PVR Polyclonal Antibody for WB, ELISA - P15151

Core Product Specifications and Parameters

Biological Background: CD155/PVR Function and Localization

• CD155, also known as Poliovirus receptor (PVR) or Nectin-like protein 5 (NECL-5), is a transmembrane glycoprotein belonging to the nectin family of cell adhesion molecules. It is encoded by the PVR gene and undergoes alternative splicing to produce multiple isoforms.
• CD155 mediates NK cell adhesion and triggers effector functions by interacting with NK cell receptors CD96 and CD226, forming immunological synapses that promote cytotoxicity and IFN-gamma secretion. This interaction is critical for innate immune surveillance.
• As a poliovirus receptor, CD155 facilitates viral entry and axonal transport by linking virion-containing endocytic vesicles to the microtubule network via DYNLT1. It also serves as a receptor for Pseudorabies virus.
• CD155 plays a role in tumor cell invasion and migration, and its overexpression in some cancers can promote fratricide NK cell activation, potentially aiding immune evasion.
• Subcellularly, CD155 is localized primarily at the cell membrane, but a secreted form also exists, likely generated by alternative splicing or proteolytic cleavage.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which regulate its function and stability.
• The protein has a molecular weight of approximately 45 kDa (45303 Da as per UniProt), consistent with the product specification.
• CD155’s interactions and functions make it a key molecule in immuno-oncology and virology research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the extracellular domain of CD155 (amino acids 220–345), which spans the second and third immunoglobulin-like domains. This design targets conserved epitopes likely present in both human and mouse CD155, consistent with the reported cross-reactivity. Consider validating specificity in your sample system using positive and negative controls.
• For Western Blot (WB), the predicted molecular weight is ~45 kDa. Optimize lysis conditions to maintain membrane protein integrity, and consider using a mild detergent to preserve epitopes. Verify the band size against known standards.
• For ELISA, this antibody can be used for direct or indirect detection of CD155 in cell lysates or purified protein. Titrate the antibody to determine optimal working concentrations for your assay format.
• Given the antibody’s reactivity with mouse CD155, it may be suitable for cross-species studies, but independent validation in mouse models is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing premium IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For researchers and diagnostic developers focused on immunology and oncology, this anti-CD155 antibody supports NK cell and tumor biology studies. Whether you need optimized monoclonal/polyclonal antibodies, rare target raw materials, or technical consulting, CamelBio streamlines your path from concept to clinic.