Anti-CD155 (PVR/NECL-5) Rabbit Polyclonal Antibody for WB and ELISA - P15151

Core Product Specifications and Parameters

Biological Background: CD155 (PVR) Function and Localization

• CD155, also known as poliovirus receptor (PVR) or nectin-like protein 5 (NECL-5), is a member of the immunoglobulin superfamily involved in cell adhesion and immune recognition.
• The protein mediates natural killer (NK) cell adhesion and triggers effector functions by binding to two NK cell receptors, CD96 and CD226, leading to the formation of immunological synapses and the release of cytotoxic granules and IFN-gamma.
• CD155 plays a role in tumor cell invasion and migration, and its high expression on some tumors may enable immune evasion through fratricide NK cell activation or modular exchange of PVR molecules.
• As a crucial host cell receptor, CD155 facilitates the entry of poliovirus by interacting with the virion and targeting endocytic vesicles to the microtubular network via DYNLT1 for axonal retrograde transport. It also acts as a receptor for Pseudorabies virus.
• Human cytomegalovirus (HCMV) infection prevents CD155 from reaching the cell surface, likely as a mechanism to evade NK cell-mediated immune recognition.
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and exists as multiple isoforms generated by alternative splicing.
• Subcellularly, CD155 is primarily located at the cell membrane, but a secreted form also exists, suggesting dual functions in both cell-bound and soluble forms.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against the extracellular region of human CD155, may recognize multiple isoforms. For Western blot analysis, expect bands between 39 and 45 kDa depending on cell type and post-translational modifications; validate in your specific cell lysate.
• For ELISA, consider pairing this antibody with a monoclonal CD155 capture antibody to establish a sensitive sandwich assay. Titration and optimization of antibody concentration are recommended.
• Since CD155 is expressed on target cells and can be shed as a secreted form, sample preparation should account for both membrane-bound and soluble protein pools.
• The antibody is validated for human CD155; cross-reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable, high-quality raw-material sourcing solutions for immuno-oncology, virology, and cell adhesion targets. As a one-stop supplier, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like CD155. Let CamelBio accelerate your assay development from concept to clinic.