Anti-CD153 Monoclonal Antibody for Flow Cytometry - P32971

Core Product Specifications and Parameters

Biological Background: CD153 (CD30L/TNFSF8) Function and Localization

• Tumor necrosis factor ligand superfamily member 8 (TNFSF8), also known as CD30 ligand (CD30-L) or CD153, is encoded by the TNFSF8 gene in humans.
• CD153 binds to its receptor TNFRSF8/CD30 and acts as a cytokine that induces proliferation of T-cells.
• It is a type II transmembrane glycoprotein, anchored to the plasma membrane via a signal-anchor sequence, with an extracellular C-terminal receptor-binding domain.
• The protein contains a disulfide bond and is glycosylated, characteristic of TNF ligand family members.
• As a membrane-bound cytokine, CD153 is involved in cell-cell communication within the immune system.
• The extracellular domain (amino acids 63-234) used for immunization corresponds to the mature soluble form after cleavage, but the full-length protein functions primarily as a transmembrane ligand.
• CD153 is a reference proteome member identified through proteomics studies.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is recommended for flow cytometry (FC). Titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions.
• Since the immunogen covers the extracellular region (aa 63-234), the antibody likely recognizes the native conformation of CD153 on live, unfixed cells. Permeabilization may be required for intracellular staining; validate in your relevant sample system.
• Include appropriate isotype controls to account for non-specific binding and background in your flow cytometry experiments.
• CD153 expression is often upregulated on activated T cells; therefore, consider stimulation protocols to enhance detection in resting lymphocyte populations.
• Avoid repeated freeze-thaw cycles and store the antibody as recommended. Protect from light to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

• As a dedicated IVD raw-material partner, CamelBio bridges the gap from concept to clinic by offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents for diagnostic development.
• This anti-CD153 monoclonal antibody exemplifies our commitment to providing high-quality, application-validated products for immunology and disease biomarker research, supporting your flow cytometry needs.
• For custom bulk orders, rare target antibody development, or optimized reagent sourcing, our team provides tailored solutions to accelerate your assay development and manufacturing processes.