Anti-CD152/CTLA-4 Polyclonal Antibody for WB, ELISA - P16410

Core Product Specifications and Parameters

Biological Background: CTLA-4 Function and Localization

• CTLA-4 (Cytotoxic T-lymphocyte protein 4) is an inhibitory receptor and member of the immunoglobulin superfamily that serves as a major negative regulator of T-cell immune responses.
• It acts as a decoy receptor: its affinity for B7 ligands CD80 and CD86 is considerably stronger than that of the stimulatory coreceptor CD28, thereby outcompeting CD28 and dampening T-cell activation. Related references: PMID:11279501 PMID:11279502 PMID:16551244
• The CTLA-4 protein is a single-pass transmembrane glycoprotein localizing predominantly to the cell membrane, with its extracellular domain containing a V-type immunoglobulin fold.
• Although present in various tissues, CTLA-4 is most notably expressed in activated T-cells, where its surface levels are 30- to 50-fold lower than CD28, ensuring tight regulation of co-inhibitory signals.
• Alternative splicing generates multiple isoforms, and the protein undergoes post-translational modifications including glycosylation and phosphorylation, which modulate its trafficking and signaling.
• Genetic variations in CTLA4 are associated with autoimmune disorders such as type 1 diabetes (IDDM12), systemic lupus erythematosus, and autoimmune lymphoproliferative syndrome (ALPS5).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 49-223), so this polyclonal antibody likely recognizes native and denatured CTLA-4. For western blot, expect a band at approximately 25 kDa; consider using activated T-cell lysates or CTLA-4-overexpressing cells as positive controls.
• For ELISA, antigen-down or sandwich formats may be feasible; validate the antibody in the relevant sample system and matrix to confirm performance.
• Since the antibody cross-reacts with mouse and rat, it can be used for cross-species studies, but always verify reactivity in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CTLA-4 polyclonal antibody is well-suited for immuno-oncology and autoimmune disease research, supporting the development of assays that target T-cell checkpoint pathways. With a commitment to bridging concept-to-clinic needs, CamelBio provides tailored sourcing and technical consulting to streamline your IVD development workflows.