Anti-CD151 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P48509

Core Product Specifications and Parameters

Biological Background: CD151 Function and Localization

• CD151 antigen, also known as PETA-3, TSPAN24, and SFA-1, is a member of the tetraspanin family, characterized by four transmembrane domains, two extracellular loops, and short intracellular termini.
• As a structural component of tetraspanin-enriched microdomains (TERMs), CD151 facilitates receptor clustering and signaling via association with integrins (e.g., ITGA3:ITGB1, ITGA6:ITGB4), regulating cell adhesion, migration, and angiogenesis (PubMed:17045834, PubMed:24723389, PubMed:31488507). Related references: PMID:17045834 PMID:24723389 PMID:31488507
• Through interaction with JAM-A/F11R and ITGA3:ITGB1, CD151 recruits RAC1, CDC42, and RhoGTPases to promote epithelial cell polarization and actin cytoskeleton reorganization, critical for cell motility (PubMed:22843693, PubMed:35067832). Related references: PMID:22843693 PMID:35067832
• CD151 modulates the glycosylation pattern of ITGA3:ITGB1, thereby influencing integrin activity (PubMed:18852263). Related references: PMID:18852263
• Essential for maintaining central laminin-binding integrin ITGA6:ITGB4-containing adhesion complexes, contributing to proper assembly of glomerular and tubular basement membranes in the kidney (PubMed:15265795, PubMed:31488507). Related references: PMID:15265795 PMID:31488507
• Expressed in vascular endothelium, epidermis, erythroid cells, and T-cells; acts as a sensitive T-cell activation marker (PubMed:32978478). Related references: PMID:32978478
• Involved in host-virus interactions, playing a role in endocytosis of human papillomavirus 16 (HPV-16) and entry of human cytomegalovirus into host cells.
• Post-translational modifications include palmitoylation and ubiquitination, and the protein is annotated with keywords: Blood group antigen, Host-virus interaction, Lipoprotein, Palmitate, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the major extracellular loop (aa 113-221) of human CD151, which is highly conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting (WB), a band at approximately 28 kDa is expected, though glycosylation may cause slight shifts; optimize protein loading and transfer conditions.
• In immunohistochemistry (IHC-P), validate staining patterns in known CD151-expressing tissues such as vascular endothelium and epidermis; include appropriate controls to confirm specificity.
• For ELISA, consider using the antibody in both direct and capture formats, and validate with recombinant CD151 protein or cell lysates from human, mouse, or rat sources.
• Since the antibody is polyclonal, it may recognize multiple epitopes; be aware of potential batch-to-batch variability and verify performance in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined, single-source solution for IVD raw materials, covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, with expertise in rare and emerging targets such as tetraspanins. For CD151, we can support your assay development with high-quality reagents and technical consultation, ensuring rapid scalability for cell adhesion and oncology diagnostic applications.