Anti-CD150/SLAM Polyclonal Antibody for WB and ELISA - Q13291

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• CD150/SLAMF1 is a self-ligand receptor of the SLAM family, modulating activation and differentiation of a wide range of immune cells, and connecting innate and adaptive immune responses.
• In T-cells, SLAMF1 signaling involves both SH2D1A-dependent and PTPN11-dependent pathways, with SH2D1A recruiting FYN to phosphorylate SLAMF1 (PubMed:12458214). Additionally, SH2D1A prevents binding to inhibitory effectors like INPP5D (PubMed:11806999). Related references:
  PMID:12458214 PMID:11806999
• SLAMF1 inhibits CD40-induced signal transduction in monocyte-derived dendritic cells (PubMed:16317102). Related references:
  PMID:16317102
• In B-cells, SLAMF1 activates ERK signaling independently of SH2D1A via SYK and INPP5D, and also activates p38 MAPK, JNK1, and JNK2 (PubMed:20231852). Related references:
  PMID:20231852
• SLAMF1 is involved in the innate immune response against Gram-negative bacteria by recognizing OmpC/F, regulating phagosome maturation, and recruiting PI3K complex II (PubMed:20818396). Related references:
  PMID:20818396
• Constitutively expressed on memory T-cells and a proportion of B-cells, and rapidly induced on naïve T-cells after activation (PubMed:7617038). Activated B-cells express isoform 1, 3, and a cytoplasmic isoform (PubMed:9091591). Isoform 4 is expressed in tumors of the central nervous system (PubMed:25710480). Related references:
  PMID:7617038 PMID:9091591 PMID:25710480
• SLAMF1 acts as a receptor for Measles virus, with isoform 4 also mediating entry.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein, amino acids 21–237 of human CD150/SLAM) corresponds to the extracellular domain, which is expected to generate antibodies recognizing the native, folded protein in assays like ELISA. For Western blot, use reducing conditions; the predicted molecular weight is ~37 kDa (monomer), although glycosylation may shift the band.
• Cross-reactivity with mouse indicates conserved epitopes; consider verifying in the relevant experimental system.
• Other applications beyond WB and ELISA may be possible but require end-user optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive suite of IVD raw materials and services, streamlining your diagnostic development from concept to clinic. This anti-CD150/SLAM antibody exemplifies our capability to supply high-quality immunological reagents for immune-oncology and infectious disease research. We also provide validated antibody pairs, bulk ancillary reagents, and rare target sourcing to support your assay manufacturing needs.