Anti-CD150/SLAM Monoclonal Antibody for FC - Q9QUM4

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• Encoded by the Slamf1 gene in mice, CD150/SLAM (signaling lymphocytic activation molecule family member 1) is a self-ligand receptor of the SLAM family that modulates activation and differentiation of a wide variety of immune cells (PubMed:9126961, PubMed:12351401). Related references: PMID:9126961, PMID:12351401
• Operates at the interface of innate and adaptive immunity; SLAM receptor signaling is controlled by cytoplasmic adapters SH2D1A/SAP and SH2D1B/EAT-2, leading to distinct signal-transduction events in T‑lymphocytes versus B‑cells.
• Promotes IL‑2‑independent T‑cell proliferation and IFN‑γ production; downstream signaling involves INPP5D, DOK1, DOK2, PRKCQ, BCL10, and NFKB1 (PubMed:11477403, PubMed:15539155, PubMed:16847311). Related references: PMID:11477403, PMID:15539155, PMID:16847311
• Enhances T‑cell receptor‑induced IL‑4 secretion by CD4⁺ cells and is required for IL‑4 production in germinal center T follicular helper cells (PubMed:15123745, PubMed:20525889). Related references: PMID:15123745, PMID:20525889
• In B‑cells, activates ERK and Akt signaling pathways independently of SH2D1A, implicating both SYK and INPP5D (PubMed:15315965). Related references: PMID:15315965
• Subcellular localization is at the cell membrane; it is a single‑pass type I transmembrane glycoprotein with immunoglobulin domains, disulfide bonds, and phosphorylation sites.
• Plays a direct role in innate immune defense against Gram‑negative bacteria in macrophages, recognizing bacterial OmpC/OmpF and regulating phagosome maturation (PubMed:20818396, PubMed:22493499). Related references: PMID:20818396, PMID:22493499

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 25–242 of mouse CD150, which includes the entire extracellular domain. Consider using this antibody for flow cytometry to detect surface expression on primary mouse immune cells.
• For FC, optimize cell preparation and staining protocols using appropriate isotype controls. Validate expression in known CD150⁺ populations such as activated T lymphocytes, B cells, or macrophages.
• As CD150 expression can be modulated by activation status, consider stimulating cells (e.g., with anti-CD3/CD28 or LPS) to upregulate target levels before staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and IVD developers by providing reliable, research‑grade raw materials backed by deep immunological expertise. This anti‑CD150 monoclonal antibody exemplifies our commitment to delivering key immune‑cell markers for flow cytometry applications. Beyond catalog products, we offer validated antibody pairs, optimized monoclonal/polyclonal panels, ancillary bulk reagents, and custom sourcing for rare target reagents—streamlining your path from concept to clinic.