Anti-CD147/BSG Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P35613

Core Product Specifications and Parameters

Biological Background: CD147/BSG Function and Localization

• Basigin (BSG/CD147/EMMPRIN) is a highly glycosylated transmembrane protein belonging to the immunoglobulin superfamily; multiple isoforms are generated by alternative splicing and promoter usage.
• Acts as a signaling receptor for cyclophilins (PPIA/CYPA and PPIB/CYPB), mediating chemotaxis and adhesion of immune cells. Related references: PMID:11688976 PMID:11943775
• Essential for the plasma membrane targeting of monocarboxylate transporters (SLC16A1, SLC16A11, SLC16A12), regulating lactate transport and cellular metabolism. Related references: PMID:17127621 PMID:21778275 PMID:28666119
• Functions as a co-receptor for VEGFR2 (KDR) in endothelial cells, enhancing VEGF-mediated angiogenesis and promoting tumor growth, invasion, and metastasis through matrix metalloproteinase induction. Related references: PMID:25825981 PMID:19837976 PMID:11992541 PMID:12553375 PMID:15833850
• Plays a critical role in retinal maturation and cone photoreceptor survival by acting as a receptor for NXNL1, enhancing aerobic glycolysis. Related references: PMID:25957687
• Serves as a receptor for pathogens: erythrocyte receptor for Plasmodium falciparum RH5 (essential for merozoite invasion) and facilitates entry of SARS-CoV, HIV-1, measles virus, and HCMV; not required for SARS-CoV-2 infection. Related references: PMID:22080952 PMID:26195724 PMID:28409866
• Subcellularly localized to the cell membrane, melanosomes, photoreceptor segments, endosomes, and endoplasmic reticulum. Highly expressed in retina, erythrocytes, heart, kidney, skeletal muscle, testis, bone marrow, fetal liver, lung, and thymus. Related references: PMID:25957687 PMID:26195724 PMID:28409866 PMID:11992541 PMID:21536654

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 286-385) of human CD147, which is highly conserved across human, mouse, and rat, consistent with the observed cross-reactivity. Validate reactivity in target species and tissue.
• For Western blotting (WB), the predicted molecular weight is ~42 kDa, but extensive glycosylation may result in a higher apparent molecular weight (approx. 45–65 kDa). Use appropriate positive controls and consider deglycosylation if needed.
• In immunohistochemistry (IHC-P), antigen retrieval and blocking steps may significantly impact staining; test with known positive tissues such as melanoma or retinal sections.
• For immunoprecipitation (IP), optimize antibody-to-lysate ratios and validate elution conditions; note that the target is a membrane protein, so include appropriate detergents.
• For ELISA, consider direct or capture formats; pairing with another CD147 antibody may be needed for sandwich ELISA. Validate using recombinant protein or cell lysates.
• As a polyclonal antibody, lot-to-lot consistency may vary; always include proper internal controls and re-validate performance with each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers, offering one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-CD147/BSG polyclonal antibody supports research and assay development in oncology, immunology, and infectious disease. Contact us for custom bulk orders, technical consultation, and project-specific solutions tailored to your IVD pipeline.