Anti-CD147/BSG Rabbit Monoclonal Antibody for WB and ELISA - P35613

Core Product Specifications and Parameters

Biological Background: CD147/BSG Function and Localization

• Basigin (CD147/EMMPRIN) is a multifunctional transmembrane glycoprotein belonging to the immunoglobulin superfamily, serving as a signaling receptor for cyclophilins and a chaperone for monocarboxylate transporters.
• Essential for retinal maturation and cone photoreceptor survival by acting as a receptor for NXNL1, enhancing aerobic glycolysis, and accelerating glucose entry (PubMed:25957687).
  Related references: PMID:25957687
• Plays a key role in tumor invasion, metastasis, and angiogenesis by stimulating the production of extracellular matrix metalloproteinases and upregulating VEGFA/VEGFR2 signaling in tumor and stromal cells (PubMed:11992541, 12553375, 15833850).
  Related references: PMID:11992541, PMID:12553375, PMID:15833850
• Acts as a chaperone for glucose transporter SLC16A1/GLUT1 and other SLC16A members, targeting them to the plasma membrane (PubMed:17127621, 21778275, 28666119).
  Related references: PMID:17127621, PMID:21778275, PMID:28666119
• Functions as a receptor for cyclophilins PPIA/CYPA and PPIB/CYPB, mediating chemotaxis and adhesion of immune cells (PubMed:11688976, 11943775).
  Related references: PMID:11688976, PMID:11943775
• Serves as an erythrocyte receptor for Plasmodium falciparum RH5, essential for merozoite invasion, and acts as a receptor for measles virus and human cytomegalovirus entry into epithelial and endothelial cells (PubMed:22080952, 26195724, 28409866).
  Related references: PMID:22080952, PMID:26195724, PMID:28409866
• Subcellular localization includes the cell membrane, melanosomes, endosomes, endoplasmic reticulum, and photoreceptor segments, reflecting its diverse functions.
• Highly expressed in retina, erythrocytes, melanoma cell lines, and tissues like heart, kidney, skeletal muscle, testis, bone marrow, fetal liver, lung, and thymus.

Experimental Guidance and Technical Tips

• The immunogen peptide (amino acids 100–200) targets the extracellular Ig-like domain, likely conserved between human and mouse, supporting cross-reactivity.
• For Western blot, note that CD147/BSG exists as multiple glycosylated forms (19–42 kDa); deglycosylation may be used to confirm the core protein size.
• Optimize blocking and antibody dilution conditions for each application and sample type; validate with positive and negative controls in your specific experimental system.
• In ELISA, test different coating buffers and consider a sandwich format with a paired detection antibody to enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. The CD147/BSG rabbit monoclonal antibody supports research into tumor invasion, angiogenesis, and microbial pathogenesis, and can be sourced alongside validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. Whether you require rare-target sourcing or streamlined procurement, CamelBio bridges your IVD supply chain needs.