Anti-CD147 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P18572

Core Product Specifications and Parameters

Biological Background: Basigin (CD147) Function and Localization

• Basigin (CD147), also known as HT7 antigen, EMMPRIN, or gp42, is a transmembrane glycoprotein of the immunoglobulin superfamily encoded by the Bsg gene (UniProt: P18572).
• It is essential for normal retinal maturation and development, acting as a receptor for NXNL1 and promoting cone photoreceptor survival by enhancing aerobic glycolysis with SLC16A1/GLUT1. Related references: PMID:10967074 PMID:11273674 PMID:11853760 PMID:25957687
• Functions as a signaling receptor for cyclophilins (PPIA/CYPA, PPIB/CYPB) and is involved in chemotaxis and adhesion of immune cells (By similarity).
• Chaperones the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane, a role critical for cellular metabolism. Related references: PMID:12601063
• In endothelial cells, CD147 acts as a coreceptor for KDR/VEGFR2, enhancing VEGFA-mediated signaling and promoting angiogenesis (By similarity).
• Plays a role in spermatogenesis by mediating germ cell–Sertoli cell interactions and is essential for germ cell differentiation. Related references: PMID:11882021 PMID:23727514
• Contributes to neurite outgrowth in the nervous system.
• Highly expressed in retina, testis, brain, lung, liver, kidney, heart, and spleen, with specific localization to photoreceptor inner and outer segments, cell membrane, and endoplasmic reticulum. Related references: PMID:11273674 PMID:11853760 PMID:12939332 PMID:25957687 PMID:12601063 PMID:2361961
• Post-translationally modified by glycosylation and phosphorylation, and contains disulfide bonds and a mannose-binding lectin-like domain.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 141–302) corresponds to the extracellular region of mouse CD147, suggesting that the antibody recognizes the native protein.
• For Western blot, a band at approximately 42 kDa is expected; use mouse tissue or cell lines known to express CD147 (e.g., retina, testis) with appropriate loading controls.
• In IHC-P, optimize antigen retrieval (e.g., heat-induced epitope retrieval in citrate buffer) and validate with tissue sections expressing CD147, such as mouse retina or testis.
• For ELISA, the antibody can be used for detection or capture of mouse CD147; consider pairing with a secondary antibody specific to rabbit IgG.
• As a rabbit monoclonal antibody, it offers high specificity and consistency; confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive B2B partner for IVD raw-material sourcing, offering validated antibody pairs, bulk ancillary reagents, and rare target materials to support your entire product development cycle. For targets like CD147 (basigin) involved in retinal biology, immune signaling, and transporter research, we provide ready-to-use monoclonal antibodies and customized sourcing solutions to accelerate your assay development and diagnostic innovation.