Anti-CD146/MCAM Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P43121

Core Product Specifications and Parameters

Biological Background: CD146/MCAM Function and Localization

• CD146, also known as MUC18, MEL-CAM, or S-endo 1, is a cell adhesion molecule belonging to the immunoglobulin superfamily.
• It contributes to endothelial monolayer cohesion at intercellular junctions and may facilitate melanoma cell interaction with vascular elements, enhancing hematogenous metastasis.
• Acts as a surface receptor that triggers intracellular signaling upon engagement, including tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient calcium influx.
• The protein is a single-pass type I membrane glycoprotein composed of a signal peptide, five Ig-like domains, a transmembrane helix, and a cytoplasmic region.
• Expressed predominantly in vascular endothelial cells and smooth muscle; upregulated in advanced primary melanomas and metastatic lesions, but rarely detected in benign nevi.
• Post-translational modifications include N-linked glycosylation and stimulus-induced tyrosine phosphorylation.

Experimental Guidance and Technical Tips

• As the immunogen (aa 547-646) lies within the cytoplasmic domain, permeabilization is recommended for IHC-P and cell-surface-inaccessible ELISA formats.
• For Western blotting, a band at approximately 72 kDa is expected under reducing conditions; validate specificity with positive (e.g., HUVEC lysate) and negative controls.
• When performing IHC-P, optimize antigen retrieval and antibody dilution; consider using a polymer-based detection system for sensitivity.
• The antibody's cross-reactivity with mouse and rat offers flexibility for translational studies; verify performance in the relevant species' tissue.
• For ELISA, confirm that the epitope is available in the assay format; direct antigen coating may require denaturation to expose the peptide sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of validated IVD raw materials, including this CD146/MCAM monoclonal antibody for vascular and cancer biology applications. We supply optimized antibody pairs, bulk ancillary reagents, and offer customized sourcing for rare-target raw materials—supporting your workflow from initial R&D to commercial kit assembly. Partner with us for reliable, end-to-end procurement solutions.