Anti-CD146/MCAM Monoclonal Antibody for WB, IF/ICC, ELISA - P43121

Core Product Specifications and Parameters

Biological Background: CD146/MCAM Function and Localization

• Encodes a cell surface glycoprotein involved in cell adhesion and endothelial monolayer cohesion at intercellular junctions in vascular tissue.
• Acts as a surface receptor triggering tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in intracellular calcium concentration.
• Enhances hematogenous tumor spread; expression is associated with melanoma progression and metastasis, detected strongly on metastatic lesions and advanced primary tumors.
• Localized to the plasma membrane (single-pass type I membrane protein); detected in endothelial cells, vascular smooth muscle, and neural crest cells during embryonic development.
• Contains immunoglobulin-like domains and belongs to the immunoglobulin superfamily; undergoes alternative splicing producing multiple isoforms.
• Post-translational modifications include phosphorylation, N-linked glycosylation, and formation of disulfide bonds, as supported by UniProt annotations.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 24-559) covers the extracellular Ig-like domains, enabling detection of both full-length membrane-bound and potential soluble forms.
• For WB, consider a molecular weight of approximately 72 kDa; note that glycosylation may cause a higher apparent molecular weight in some cell types.
• For IF/ICC, expect membrane staining; permeabilization may be required for optimal access if targeting intracellular epitopes.
• For ELISA, the antibody recognizes human CD146/MCAM; validate specificity using recombinant protein or cell lysates in the relevant sample system.
• Cross-reactivity is reported as human; when working with other species, verify specificity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide one-stop IVD raw-material sourcing for targets like CD146/MCAM. We supply validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials—supported by technical services spanning concept to clinic. Explore our portfolio for reliable, high-performance antibodies and enrich your cell adhesion and cancer research pipelines.