Anti-CD146 Polyclonal Antibody for WB, ELISA - Q8R2Y2

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• CD146 (also known as MUC18 or gicerin) is a cell surface glycoprotein encoded by the Mcam gene, with alternative splicing generating multiple isoforms.
• Functions as a cell adhesion molecule, contributing to endothelial monolayer cohesion at intercellular junctions in vascular tissue.
• Its expression on melanoma cells may facilitate interactions with the vascular system, potentially enhancing metastatic spread.
• Participates in neural crest cell migration during embryonic development.
• Acts as a surface receptor, triggering tyrosine phosphorylation of FYN and PTK2/FAK and a transient increase in intracellular calcium (by similarity).
• Located at the plasma membrane as a single-pass type I membrane protein.
• Contains immunoglobulin-like domains; undergoes glycosylation, phosphorylation, and disulfide bond formation.
• Detected in melanoma cell lines.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 24–563, covering the majority of the extracellular domain; the antibody is expected to recognize the extracellular region of CD146.
• For Western blotting, a band at approximately 71 kDa (calculated from sequence) is expected; validate under reducing and non-reducing conditions as needed.
• Cross-reactivity has been confirmed with human and mouse samples; consider performing additional validation if testing other species.
• This polyclonal antibody may be suitable for ELISA; appropriate pairing with an anti-rabbit secondary antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for sourcing IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. For researchers focusing on cell adhesion, melanoma, or vascular biology, we can supply this anti-CD146 antibody along with technical support and consulting services tailored to your assay development needs. Let us bridge your raw material requirements from concept to clinic.