Anti-CD146 Polyclonal Antibody for WB, ELISA - P43121

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• Cell surface glycoprotein MUC18 (CD146), encoded by the MCAM gene, is also known as melanoma cell adhesion molecule, MUC18, HEMCAM, METCAM, MelCAM, and S-endo 1 endothelial-associated antigen.
• It functions as a cell adhesion molecule that plays a critical role in maintaining endothelial monolayer cohesion at intercellular junctions in vascular tissue.
• Its expression may facilitate melanoma cell interaction with vascular elements, promoting hematogeneous tumor spread and metastasis.
• Acts as a surface receptor triggering tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in intracellular calcium concentration.
• May be involved in neural crest cell migration during embryonic development.
• Predominantly localized to the membrane; tissue expression is detected in endothelial cells throughout the vasculature and in vascular smooth muscle of normal adult tissues.
• Strongly expressed in metastatic lesions and advanced primary melanomas, but rarely detected in benign melanocytic nevi and thin primary melanomas with low metastatic probability.
• Classified as an immunoglobulin domain-containing glycoprotein, with features including disulfide bonds, phosphorylation, and alternative splicing.

Experimental Guidance and Technical Tips

• As the antibody is raised against a peptide within the C-terminal region (a.a. 546–645), consider validating its reactivity in relevant sample systems.
• For Western blot, theoretical molecular weight is ~72 kDa; ensure appropriate gel percentage and transfer conditions.
• For ELISA, optimization of coating antigen and antibody dilution is recommended.
• Cross-reactivity with mouse and rat has been observed; verify species-specific detection in multi-species experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-CD146 polyclonal antibody supports studies in cell adhesion, melanoma metastasis, and vascular biology. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare target raw materials.