Anti-CD144/VE Cadherin Monoclonal Antibody for FC - P33151

Core Product Specifications and Parameters

Biological Background: CD144/VE Cadherin (Cadherin-5) Function and Localization

• Cadherin-5, also known as VE-cadherin or 7B4 antigen, is a calcium-dependent cell adhesion protein encoded by the CDH5 gene. It is a member of the cadherin superfamily and functions primarily as a homophilic adhesion receptor.
• It mediates calcium-dependent homophilic cell adhesion, contributing to the sorting of heterogeneous cell types. Related references: PMID:21269602
• Essential for endothelial cell cohesion and organization of intercellular junctions, it associates with alpha-catenin to anchor the actin cytoskeleton. Related references: PMID:10861224
• Together with KRIT1 and PALS1, it establishes and maintains correct endothelial cell polarity and vascular lumen by recruiting and activating the Par polarity complex and RAP1B. Related references: PMID:20332120
• Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechanotransduction. Related references: PMID:25795300
• Associates with CTNND1/p120-catenin to control CADH5 endocytosis, a mechanism that regulates cadherin surface levels.
• It localizes to cell junctions, specifically adherens junctions, as well as the cell membrane and cytoplasm.
• Expression is predominantly in endothelial cells (at the protein level) and also in the brain. Related references: PMID:27338829 ; PMID:2059658
• The protein is a glycoprotein and undergoes phosphorylation, as indicated by its keyword annotations, suggesting regulated signaling functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 48-593 of human CD144/VE Cadherin, spanning the extracellular domain, making this antibody well-suited for detecting surface epitopes on live cells by flow cytometry.
• As a directly conjugated antibody (), it can be used immediately without a secondary staining step. Prior to critical experiments, titrate the antibody to establish optimal concentrations for your specific cell types.
• Validate staining specificity using appropriate isotype controls and include positive (e.g., primary endothelial cells or HUVECs) and negative (e.g., CDH5-negative lines) controls.
• Consider fixation and permeabilization effects if intracellular staining is required, as the epitope is extracellular; test under both live and fixed conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing streamlined access to validated IVD raw materials. This anti-CD144/VE Cadherin antibody supports advanced endothelial cell biology research and vascular disease studies, complementing our portfolio of high-quality monoclonal and polyclonal antibodies. We supply ready-to-use conjugated antibodies, antibody pairs for assay development, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your project from concept to clinic.