Anti-CD144 Rabbit Polyclonal Antibody for WB, ELISA - P55284

Core Product Specifications and Parameters

Biological Background: Cadherin-5 Function and Localization

• Cadherin-5 (VE-cadherin) is a classical cadherin encoded by the Cdh5 gene, playing a calcium-dependent cell adhesion role that contributes to sorting of heterogeneous cell types and maintenance of endothelial intercellular junctions. Related references: PMID:20332120 PMID:9220534
• It associates with alpha-catenin forming a link to the cytoskeleton, and acts as a cell junctional complex anchor for AMOTL2 and MAGI1 to couple actin fibers to cell junctions. Related references: PMID:24806444
• Cadherin-5 cooperates with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen formation. Related references: PMID:27466317
• Its association with CTNND1/p120-catenin controls endocytosis, regulating cadherin turnover and junctional dynamics. Related references: PMID:33972531
• Subcellular localization includes the cell junction, adherens junction, cell membrane, and cytoplasm.
• Post-translational modifications include cleavage on pair of basic residues, glycosylation, and phosphorylation, highlighting its regulatory complexity.
• It is expressed in postnatal endothelial cells of the retinal vascular plexus at the protein level, underscoring its role in vascular development.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from mouse endothelial-rich tissues or cultured endothelial cells. Optimize antibody dilution and blocking conditions to achieve optimal signal-to-noise ratios.
• In ELISA applications, the polyclonal antibody can be employed as either capture or detection reagent; validate pairing and assay conditions in the relevant experimental system.
• Since the immunogen spans a large extracellular region (amino acids 46–592), this antibody may recognize different isoforms or cleavage products; confirm specificity in your sample and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to raw materials for IVD development, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Our CD144/VE-cadherin rabbit polyclonal antibody supports research into endothelial biology, vascular development, and cell adhesion. For large-scale procurement or sourcing of rare targets, contact CamelBio to streamline your supply chain.