Anti-CD142 (Tissue Factor) Monoclonal Antibody for IF/ICC, FC - P13726

Core Product Specifications and Parameters

Biological Background: Tissue Factor Function and Localization

• CD142, also known as Tissue Factor (TF), Coagulation factor III, or Thromboplastin, is a transmembrane glycoprotein that serves as the primary initiator of the extrinsic blood coagulation cascade.
• Upon vascular injury, TF forms a high-affinity complex with circulating factor VII (FVII) or its active form VIIa, and the TF:VIIa complex proteolytically activates factor IX and factor X, leading to thrombin generation and fibrin clot formation.
• TF plays a critical role in normal hemostasis by assembling the coagulation protease cascade on cell surfaces, triggering local clot formation in response to tissue damage.
• The human F3 gene encodes a protein of 295 amino acids, with the mature protein having a molecular weight of approximately 33 kDa; alternative splicing may generate additional isoforms.
• Subcellularly, TF is predominantly localized to the plasma membrane as a single-pass type I membrane protein, but a soluble, secreted form can also be generated by alternative splicing.
• TF is highly expressed in tissues with a protective hemostatic barrier, particularly in the lung, placenta, and pancreas, consistent with its role in preventing hemorrhage.
• Post-translational modifications include disulfide bond formation in the extracellular domain, palmitoylation of the cytoplasmic tail, and N-linked glycosylation, which are important for proper function and stability.
• As a key coagulation factor, TF is categorized under keywords such as blood coagulation, hemostasis, glycoprotein, lipoprotein, and transmembrane protein.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), consider appropriate fixation and permeabilization conditions, as TF is a transmembrane protein; methanol or paraformaldehyde fixation may be tested.
• In flow cytometry (FC), titrate the antibody to determine the optimal concentration for your cell type, and include appropriate isotype controls.
• Since the immunogen spans the extracellular domain (aa 34-251), the antibody is expected to recognize the native conformation of TF on the cell surface, supporting live-cell staining applications.
• Validate the antibody in your specific sample system, as expression levels of CD142 vary across tissues; lung, placenta, or activated monocytes may serve as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-CD142 antibody supports hemostasis and coagulation studies, and we also offer sourcing solutions for rare targets and customized production to accelerate your assay development from concept to clinic.