Anti-CD141/Thrombomodulin Rabbit PolymAb® for WB, IHC-P, IF/ICC, FC, ELISA - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin Function and Localization

• Thrombomodulin (TM; CD141), encoded by the THBD gene, is a type I transmembrane glycoprotein and receptor expressed primarily on vascular endothelial cells.
• Functions as a critical cofactor for thrombin-mediated activation of protein C, triggering the anticoagulant protein C pathway and thereby regulating hemostasis. Related references: PMID:10761923, PMID:29323190, PMID:33836597, PMID:9395524
• Accelerates activation of carboxypeptidase B2 (CPB2/TAFI), which inhibits fibrinolysis by removing C-terminal basic amino acids from kinins and anaphylatoxins. Related references: PMID:26663133
• Modulates protease-activated receptor 1 (PAR1) cleavage specificity, inhibiting endothelial permeability and inflammation.
• Exerts anti-inflammatory effects distinct from its anticoagulant role by binding and sequestering HMGB1, blocking RAGE-mediated inflammatory signaling. Related references: PMID:15841214
• Post-translational modifications include glycosylation, hydroxylation, and attachment of chondroitin sulfate; contains EGF-like domains and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 19-515), suggesting the antibody may recognize both native and denatured forms; consider testing WB under reducing and non-reducing conditions.
• For IHC-P and IF/ICC, antigen retrieval may be required; validate staining patterns in vascular tissues where thrombomodulin is highly expressed in endothelial cells.
• In flow cytometry (FC), CD141/BDCA-3 serves as a marker for myeloid dendritic cells; include appropriate negative controls such as non-endothelial cell lines.
• When developing ELISA assays, validate the antibody as capture or detection reagent in relevant biological matrices, and consider pairing with detection antibodies targeting non-overlapping epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with integrated access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for hemostasis and thrombosis research. Our one-stop sourcing model streamlines procurement for rare targets like thrombomodulin, accelerating assay development from concept to clinic. Whether you need customized antibody panels or large-scale production support, CamelBio delivers reliable solutions for the IVD community.