Anti-CD141/Thrombomodulin Rabbit Monoclonal Antibody for IHC-P, IF/ICC, FC, ELISA - P07204

Core Product Specifications and Parameters

Biological Background: CD141/Thrombomodulin Function and Localization

• Thrombomodulin (also known as Fetomodulin or CD141) is an endothelial cell receptor that plays a critical role in regulating hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Related references: PMID:10761923

• It serves as an essential cofactor for thrombin-mediated activation of protein C (PROC), initiating the anticoagulant pathway on vascular endothelial cells. Related references: PMID:29323190, PMID:33836597, PMID:9395524

• Thrombomodulin accelerates the activation of plasma carboxypeptidase B2 (CPB2/TAFI), which inhibits fibrinolysis by removing C-terminal basic amino acids from substrates such as kinins and anaphylatoxins. Related references: PMID:26663133

• It suppresses inflammation independently of its anticoagulant activity by sequestering the pro-inflammatory molecule HMGB1, preventing its interaction with cellular receptors like RAGE. Related references: PMID:15841214

• Thrombomodulin also protects endothelial barrier integrity by altering protease-activated receptor 1 (PAR1) cleavage specificity, thereby inhibiting endothelial cell permeability (By similarity).

• Subcellularly, thrombomodulin is localized primarily at the plasma membrane, consistent with its receptor functions.

• The protein is predominantly expressed in endothelial cells and undergoes post-translational modifications including glycosylation, disulfide bond formation, and hydroxylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19–515), which contains all EGF-like repeats and the lectin-like domain; this design likely yields antibodies recognizing native, surface-accessible epitopes suitable for flow cytometry and immunohistochemistry.
• For IHC-P, consider testing multiple antigen retrieval methods (e.g., heat-induced epitope retrieval at pH 6 or 9) and titrating the antibody concentration due to potential variability in tissue fixation.
• For IF/ICC, include appropriate permeabilization steps if targeting intracellular pools, and validate with a negative control to confirm membrane-specific staining.
• In flow cytometry, use a live-cell staining protocol with an appropriate fluorophore-conjugated secondary antibody to minimize non-specific binding; verify performance with a known positive endothelial cell line.
• For ELISA-based detection of soluble thrombomodulin in plasma or serum, evaluate the antibody as capture or detection reagent in a sandwich format, considering potential matrix effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-CD141/Thrombomodulin rabbit monoclonal antibody alongside a broad portfolio of IVD raw materials supporting diagnostic and research workflows in coagulation and vascular biology. We offer bulk antibody supply, complementary antigen pairs, and custom sourcing services to accelerate your assay development from concept to clinic.