Anti-CD14 Rabbit Monoclonal Antibody for WB - P10810

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• Monocyte differentiation antigen CD14 (CD14), also known as myeloid cell-specific leucine-rich glycoprotein, is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein belonging to the leucine-rich repeat (LRR) family.
• CD14 acts as a coreceptor for bacterial lipopolysaccharide (LPS) in concert with LPS-binding protein (LBP), delivering LPS to the LY96/TLR4 complex to initiate innate immune responses (PubMed:16148141).
  Related references:
  PMID:16148141
• Downstream signaling via MyD88, TIRAP, and TRAF6 leads to NF-kappa-B activation, cytokine secretion, and inflammatory response (PubMed:15895089, PubMed:8612135).
  Related references:
  PMID:15895089, PMID:8612135
• CD14 serves as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides, respectively; these complexes trigger signaling from the cell surface and traffic to the Golgi in a lipid-raft dependent manner.
• As an accessory receptor, CD14 binds M.tuberculosis lipoproteins LprA, LprG, and LpqH in conjunction with TLR2 and TLR1, modulating antigen-presenting cell functions (PubMed:19362712).
  Related references:
  PMID:19362712
• CD14 also binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-).
• Subcellular localization includes the cell membrane, membrane rafts, Golgi apparatus, and it can be secreted in a soluble form.
• Detected on peritoneal macrophages, lung alveolar macrophages, dendritic macrophages, and lung macrophages at the protein level (PubMed:8612135, PubMed:19362712).
  Related references:
  PMID:8612135, PMID:19362712

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (residues 18-345) of mouse CD14, which encompasses the leucine-rich repeat region; this design can be useful for detecting both membrane-bound and soluble CD14 forms.
• For Western blotting, the predicted molecular weight of CD14 is approximately 39 kDa; however, due to glycosylation, the observed band may run slightly higher. Consider using a suitable blocking and lysis buffer to preserve epitopes.
• Flow cytometry can be applied to analyze CD14 cell surface expression on macrophages and monocytes; ensure proper gating and isotype controls for mouse samples.
• ELISA may be suitable for detecting soluble CD14 or studying LPS-binding interactions; optimize coating conditions and detection antibodies for the specific assay format.
• Validate the antibody in the relevant sample system, such as mouse peritoneal macrophages or lung macrophage populations, to confirm reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized one-stop IVD raw material supplier, CamelBio is dedicated to providing diagnostic manufacturers and research laboratories with high-quality monoclonal antibodies, recombinant proteins, and ancillary reagents. This CD14 rabbit monoclonal antibody supports research and assay development in innate immunity, infectious diseases, and inflammation. Whether you require optimized antibody pairs, bulk production, or assistance sourcing rare targets, CamelBio’s integrated supply chain and technical expertise help accelerate your project from concept to clinic.