Anti-CD13/ANPEP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• CD13/ANPEP, also known as aminopeptidase N (APN) or CD13, is a zinc-dependent metalloprotease belonging to the M1 family of aminopeptidases.
• It exhibits broad substrate specificity, playing a key role in the final digestion of peptides derived from protein hydrolysis by gastric and pancreatic proteases.
• CD13 processes various peptide hormones, including angiotensin III and IV, neuropeptides, and chemokines, and may cleave peptides bound to MHC class II molecules on antigen-presenting cells.
• It is implicated in angiogenesis, cholesterol crystallization, and potentially in amino acid transport through interaction with SLC6A19.
• CD13 acts as a host receptor for human coronavirus 229E (HCoV-229E) and mediates human cytomegalovirus (HCMV) infection.
• The protein is localized on the cell membrane, with a soluble form detectable in plasma; it contains a transmembrane helix and signal-anchor, and undergoes post-translational modifications including glycosylation, disulfide bond formation, and sulfation.
• CD13 is widely expressed in epithelial cells of the kidney, intestine, and respiratory tract, as well as in granulocytes, monocytes, endothelial cells, and synaptic membranes; elevated levels are associated with cancer.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the C-terminal region (amino acids 668-967) of human CD13/ANPEP, which should detect the full-length protein (~110 kDa).
• For Western blot (WB), consider using lysates from tissues with high CD13 expression (e.g., kidney, intestine) as positive controls; validate the anti-rabbit secondary antibody compatibility.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval conditions may need optimization for your specific sample type and fixation method.
• The antibody cross-reacts with mouse and rat CD13, making it suitable for rodent model studies; however, confirm signal specificity in your experimental system.
• For ELISA applications, the antibody can be explored as a capture or detection reagent, but appropriate pair testing and standardization are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Our anti-CD13/ANPEP rabbit polyclonal antibody supports research in protease biology, angiogenesis, and viral entry mechanisms. Partner with CamelBio for efficient procurement and technical support, streamlining your development from concept to clinic.