Anti-CD13/ANPEP Monoclonal Antibody for WB, IHC-P, FC, ELISA - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• Aminopeptidase N, also designated APN, CD13, alanyl aminopeptidase, or gp150, is a zinc-dependent type II transmembrane metalloprotease encoded by the ANPEP gene.
• Functions as a broad-specificity aminopeptidase in the final digestion of peptides derived from gastric and pancreatic proteolysis; processes peptide hormones including angiotensin III and IV, neuropeptides, and chemokines.
• May cleave peptides presented on MHC class II molecules of antigen-presenting cells, contribute to angiogenesis, promote cholesterol crystallization, and regulate amino acid transport by interacting with the SLC6A19 transporter.
• Localizes to the cell membrane and is expressed in epithelial cells of kidney, intestine, and respiratory tract, as well as on granulocytes, monocytes, endothelial cells, and certain CNS synaptic membranes; a soluble form is detectable in plasma, often elevated in cancer patients.
• Serves as a cellular receptor for human coronavirus 229E (HCoV-229E) spike glycoprotein and mediates entry of human cytomegalovirus (HCMV).
• Annotated with keywords including aminopeptidase, angiogenesis, cell membrane, host cell receptor for virus entry, metalloprotease, protease, transmembrane, and zinc.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 69-967) of human CD13, supporting detection of surface‑exposed epitopes in flow cytometry and immunohistochemistry.
• For western blot, anticipate a band around 110 kDa; validate specificity using a positive control such as kidney or intestinal tissue lysate and an appropriate molecular weight marker.
• In ELISA applications, consider pairing with a second antibody that recognizes a non‑overlapping epitope to develop a robust sandwich assay.
• Optimize antigen retrieval protocols for IHC‑P, as CD13 is a transmembrane protein, and validate staining patterns in relevant normal and tumor tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers diagnostic manufacturers, labs, and research institutes a single‑source solution for IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. Our CD13/ANPEP antibody supports assay development for aminopeptidase N as a cancer biomarker or viral receptor, with reliable batch‑to‑batch consistency and scalability. Reach out to streamline your supply chain and accelerate your project from concept to clinic with CamelBio’s expert sourcing and technical services.