Anti-CD13/ANPEP Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P15144

Core Product Specifications and Parameters

Biological Background: Aminopeptidase N Function and Localization

• Aminopeptidase N (ANPEP, CD13) is a broad-specificity zinc-dependent metalloprotease that plays a key role in the final digestion of peptides in the brush border of the intestine and kidney, also processing various peptide hormones (e.g., angiotensin III and IV), neuropeptides, and chemokines.
• The enzyme is involved in antigen presentation by cleaving peptides bound to MHC class II molecules on antigen-presenting cells, and may also regulate amino acid transport by interacting with the transporter SLC6A19.
• ANPEP functions as a receptor for human coronavirus 229E (HCoV-229E) and mediates human cytomegalovirus (HCMV) infection, highlighting its role in host-virus interactions.
• Subcellularly, it is a single-pass type II membrane protein localized to the cell membrane; a soluble form is also detected in plasma and is elevated in cancer patients.
• Tissue expression is broad, including kidney, intestine, respiratory epithelium, myeloid cells, endothelial cells, CNS synaptic membranes, and angiogenic vasculature; it is found in various tumor types and lymph node metastases.
• Post-translational modifications include extensive N-glycosylation, disulfide bond formation, and sulfation, contributing to its mature molecular weight of approximately 110 kDa.
• Additional keywords: angiogenesis, cholesterol crystallization, signal-anchor, transmembrane helix, and host cell receptor for virus entry.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central extracellular domain (aa 350-450), a region likely to be well-presented and conserved across human, mouse, and rat; consider this for cross-reactivity studies.
• For Western blot, expect a specific band around 110 kDa; validate using positive controls such as human kidney or intestine lysates, and for mouse/rat, use kidney or lung tissue extracts.
• In immunohistochemistry (paraffin), membranous staining should be observed; consider antigen retrieval (e.g., citrate buffer, pH 6.0) and test antibody dilutions starting at 1:50–1:200 on normal kidney or tumor sections.
• For immunofluorescence (paraffin), optimize fixation and permeabilization; a punctate membranous pattern is anticipated.
• ELISA applications can be developed for soluble CD13 detection; evaluate pairing with a detection antibody in a sandwich format and use recombinant protein or patient plasma for standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering validated monoclonal antibodies like this anti-CD13/ANPEP clone for studying aminopeptidase N function, viral entry, and tumor angiogenesis. Beyond primary antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting assay development from early research to clinical validation. Reach out to leverage our expertise in bringing your diagnostic solutions to market efficiently.