Anti-CD13/ANPEP Monoclonal Antibody for Flow Cytometry - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• CD13/ANPEP, also known as aminopeptidase N (AP-N) or gp150, is a 110 kDa type II transmembrane zinc metalloprotease. It serves as a broad-specificity aminopeptidase, crucial for the final digestion of peptides generated by gastric and pancreatic proteases.
• This enzyme processes various bioactive peptides, including angiotensin III and IV, neuropeptides, and chemokines, and may cleave MHC class II-bound peptides on antigen-presenting cells.
• CD13 has been implicated in angiogenesis and cholesterol crystallization, and may modulate amino acid transport by interacting with the SLC6A19 transporter.
• It acts as a receptor for human coronavirus 229E, facilitating viral entry, and also mediates human cytomegalovirus infection.
• Subcellularly, CD13 localizes to the cell membrane and is expressed in a wide range of tissues, including kidney, intestine, respiratory epithelia, and various leukocytes. Elevated levels are found in plasma and effusions of cancer patients.
• Post-translational modifications include N-glycosylation, disulfide bond formation, and sulfation, essential for its enzymatic and receptor functions.

Experimental Guidance and Technical Tips

• The antibody is provided as an conjugate, ready for direct flow cytometry without secondary detection steps.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining protocol.
• Use a 488 nm laser line for excitation and collect emission with a 530/30 bandpass filter or equivalent.
• Include appropriate isotype controls and viability dyes to ensure specific staining.
• Since the immunogen spans a large extracellular region (aa 69–967), the antibody is expected to recognize the native cell-surface protein; confirm antigen accessibility in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, supporting diagnostic manufacturers and research laboratories worldwide. As a trusted partner, we provide this high-quality anti-CD13 monoclonal antibody along with a portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Whether advancing biomarker discovery or infectious disease research, CamelBio bridges the gap from concept to clinic.