Anti-CD13/ANPEP Monoclonal Antibody for FC - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• Aminopeptidase N (ANPEP/CD13) is a broad specificity zinc-dependent metalloprotease that plays a key role in the final digestion of peptides generated by gastric and pancreatic proteases.
• It processes various peptide hormones, including angiotensin III and IV, neuropeptides, and chemokines, and may cleave peptides bound to MHC class II molecules of antigen-presenting cells.
• ANPEP is a type II transmembrane glycoprotein located on the cell membrane, functioning as an ectoenzyme with a large extracellular catalytic domain.
• It is expressed in diverse tissues: epithelial cells of kidney, intestine, and respiratory tract; myeloid cells (granulocytes, monocytes); and endothelial cells, particularly in angiogenic vasculature.
• A soluble form of CD13 is found in plasma and is elevated in cancer patients' plasma and effusions, indicating potential biomarker relevance.
• CD13 serves as a receptor for human coronavirus 229E (HCoV-229E) spike glycoprotein and mediates human cytomegalovirus (HCMV) infection, linking it to host-virus interactions.
• The protein is heavily glycosylated and contains disulfide bonds; it belongs to the M1 family of aminopeptidases and is involved in amino acid transport through interaction with SLC6A19.
• Additional keywords highlight its roles in angiogenesis, cell differentiation, and development; it is also a sulfated protein with a signal-anchor.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning the extracellular domain (aa 69-967), making it suitable for detecting native surface CD13 by flow cytometry.
• For flow cytometry, titrate the antibody optimally and include appropriate isotype controls to ensure specificity in human samples.
• Given CD13 expression on myeloid cells, this antibody can be used to identify granulocyte and monocyte populations in peripheral blood or tissue preparations.
• Because CD13 is upregulated on angiogenic endothelial cells, consider validating its expression in relevant tumor or inflammatory models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, from monoclonal antibodies like this anti-CD13 clone to ancillary reagents. Our platform simplifies sourcing for immunology, oncology, and virology applications, enabling seamless transition from concept to clinic. Whether you need validated antibody pairs, bulk reagents, or rare target raw materials, CamelBio is your trusted partner in IVD development.