Anti-CD138/Syndecan-1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P18827

Core Product Specifications and Parameters

Biological Background: CD138/Syndecan-1 Function and Localization

• CD138/Syndecan-1 is a transmembrane proteoglycan that contains both heparan sulfate and chondroitin sulfate chains.
• It links the cytoskeleton to the interstitial matrix, mediating cell adhesion and signaling processes.
• Syndecan-1 regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Related references: PMID:22660413
• Subcellular localization includes the plasma membrane, secreted extracellular matrix, and extracellular exosomes.
• Detected in placenta and fibroblasts at the protein level.
• Post-translational modifications include glycosylation with heparan sulfate/chondroitin sulfate and phosphorylation.
• It can induce its own expression in dental mesenchymal and epithelial cells via an MSX1-mediated pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 25–240), so the antibody may recognize the native protein in applications requiring extracellular epitope exposure.
• For Western blotting (WB), the expected molecular weight is ~32 kDa; reducing conditions are recommended to resolve the band clearly.
• In immunohistochemistry (IHC-P), antigen retrieval protocols (e.g., heat-induced epitope retrieval) should be optimized for the specific tissue type.
• Due to cross-reactivity with mouse, this antibody can be utilized in murine models, but pilot experiments to confirm species-specific detection are advised.
• Always validate the antibody in your specific experimental system, as performance may vary with sample preparation and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This Anti-CD138/Syndecan-1 antibody supports research in exosome biology, cancer metastasis, and matrix biology. As a one-stop sourcing partner, CamelBio connects diagnostic manufacturers and research institutions with rare target raw materials, technical services, and consulting from concept to clinic.