Anti-CD138 Monoclonal Antibody for Flow Cytometry - K7GNF5

Core Product Specifications and Parameters

Biological Background: CD138/Syndecan-1 Function and Localization

• CD138 (syndecan-1) is a cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate chains, mediating cell-matrix interactions.
• It links the cytoskeleton to the interstitial matrix, playing a key role in cell adhesion and signaling.
• CD138 participates in exosome biogenesis, cooperating with syntenin-1 (SDCBP) and ALIX (PDCD6IP) to regulate the formation of extracellular vesicles.
• In dental development, CD138 can auto-induce its own expression in mesenchymal cells and induce it in adjacent epithelial cells through an MSX1-mediated pathway.
• The protein is localized to the plasma membrane as a transmembrane protein, but a cleaved ectodomain can be secreted, and it is also present in extracellular exosomes.
• Post-translational modifications include extensive glycosylation with heparan sulfate addition, and phosphorylation, classifying it as a phosphoproteoglycan.
• CD138 is widely used as a plasma cell marker in multiple species due to its expression on plasma cells and some epithelial cells.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant protein, so this antibody likely recognizes a linear epitope; conformation-specific applications like IP may require empirical testing.
• Designed for flow cytometry (FC) in pig; appropriate cell preparations and isotype controls are critical for accurate gating.
• Since CD138 can be shed or present in exosomes, assess whether cell surface staining requires viability dye or blocking of secreted forms if analyzing from complex samples.
• Cross-reactivity is reported only in pig; for other species, validate before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for flow cytometry and other applications. This anti-pig CD138 antibody supports exosome biology, dental development, and plasma cell research, with bulk and custom sourcing available to meet your project needs. From rare target reagents to ancillary supplies, CamelBio bridges the gap from concept to clinic.