Anti-CD137 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P20334

Core Product Specifications and Parameters

Biological Background: CD137 Function and Localization

• CD137, also known as 4-1BB or TNFRSF9, is a member of the tumor necrosis factor receptor superfamily.
• It functions as a receptor for TNFSF9/4-1BBL, conveying signals that enhance CD8+ T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors.
• The receptor is expressed on the surface of activated thymocytes, splenic T cells, CD4+, and CD8+ T-cells.
• It localizes to the cell membrane and contains characteristic structural features including disulfide bonds, a signal peptide, and a transmembrane domain.
• Post-translational modifications include glycosylation, as annotated in UniProt.
• The mouse CD137 protein has a calculated molecular weight of approximately 28 kDa (UniProt theoretical weight: 27.6 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24–187) of mouse CD137, suggesting the antibody likely recognizes the ligand-binding region; consider this when designing blocking or competitive binding experiments.
• In Western blot, a band around 28–30 kDa may be observed, consistent with the predicted weight and supplier's report; post-translational modifications can cause slight shifts.
• For immunofluorescence and immunocytochemistry, expect membrane-localized staining; optimize fixation and permeabilization conditions for your specific cell type.
• For ELISA, the antibody can serve as a capture or detection reagent; we recommend titrating and pairing with appropriate antigen standards.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material sourcing partner, CamelBio delivers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers, labs, and research institutes. This anti-CD137 rabbit monoclonal antibody supports immuno-oncology and T-cell biology projects, and we can source rare-target raw materials tailored to your pipeline. Whether you need research-grade or scalable supply, count on us to bridge your concept-to-clinic journey.