Anti-CD134 Rabbit Polyclonal Antibody for WB, ELISA - P47741

Core Product Specifications and Parameters

Biological Background: CD134 Function and Localization

• CD134, also known as OX40 or TNFRSF4, is a member of the tumor necrosis factor receptor superfamily.
• It functions as a receptor for TNFSF4/OX40L/GP34, serving as a costimulatory molecule critical for long-term T-cell immunity (By similarity).
• The protein is localized to the cell membrane as a transmembrane receptor.
• Characteristic structural features include disulfide bonds, glycosylation, and a signal peptide, consistent with its role as a cell surface receptor.
• CD134 is expressed at the protein level in CD4(+) T-cells and T-helper Th17 cells, underscoring its importance in adaptive immune responses.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 20-211), likely enhancing detection of the native receptor.
• In Western blot, a band at approximately 30 kDa is anticipated; validate expression using T-cell lysates.
• For ELISA, consider using the recombinant protein as a positive control and optimize coating conditions.
• Cross-reactivity with rat enables comparative studies in rodent models; confirm reactivity in the relevant species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers comprehensive access to validated IVD raw materials, including antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Our portfolio supports immunoassay development for T-cell immunology targets like CD134/OX40 and other costimulatory molecules. Contact us to explore tailored sourcing solutions for your assay needs.