Anti-CD133 (Prom1) Rabbit Monoclonal Antibody for WB, IP, IF-P, ELISA - O54990

Core Product Specifications and Parameters

Biological Background: CD133 Function and Localization

• CD133, also known as Prominin-1, is encoded by the Prom1 gene in mouse (also designated Proml1). Alternative splicing produces multiple isoforms.
• Plays a role in cell differentiation, proliferation and apoptosis; binds cholesterol in plasma membrane microdomains and contributes to apical membrane organization.
• Functions as a key regulator of disk morphogenesis during early retinal development. Related references: PMID:19228982
• Involved in regulation of MAPK and Akt signaling pathways; in neuroblastoma cells, suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
• Localizes to the apical cell membrane, microvillus membrane, cilium, photoreceptor outer segment, endoplasmic reticulum, and ER-Golgi intermediate compartment.
• Exhibits tissue-specific expression: in adult, expressed in kidney tubules and brain ependymal layer, but not in gut, liver, lung, pituitary, adrenal, heart or spleen; in embryo, present on apical side of neuroepithelial cells, lung buds, gut, ureter buds.
• Keywords indicate post-translational modifications: glycoprotein, phosphoprotein, and acetylation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 20-107 of mouse CD133. For WB, expect a band at approximately 97 kDa. Consider using tissue or cell lysates where CD133 is known to be expressed, such as kidney or retina.
• For immunofluorescence (IF-P), targeting apical membranes or primary cilia in epithelial cells may be relevant. Validate staining patterns in the appropriate cellular context.
• In immunoprecipitation (IP) assays, ensure the antibody is compatible with the chosen lysis buffer and detection system. Pre-clearing and proper controls are recommended.
• For ELISA, pairing with an appropriate detection antibody and optimizing coating conditions will be critical. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic and research laboratories in CD133-related studies on cell differentiation, cancer stem cells, and retinal development. We source and supply validated antibody pairs, bulk ancillary reagents, and rare-target monoclonal/polyclonal antibodies—empowering your pipeline from concept to clinic. Contact us to streamline your CD133 raw material procurement.