Anti-CD132/IL-2 R gamma Rabbit Monoclonal Antibody for WB, ELISA - P31785

Core Product Specifications and Parameters

Biological Background: CD132/IL-2 R gamma Function and Localization

• CD132, also known as IL-2 R gamma (IL-2RG) or common gamma chain (γc), is a cytokine receptor common subunit that pairs with multiple interleukin receptors to mediate signaling. It is encoded by the IL2RG gene in humans.
• As a shared receptor component, CD132 participates in the formation of high-affinity receptors for interleukins such as IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, playing essential roles in immune cell development and function.
• In association with IL15RA, CD132 is involved in the stimulation of neutrophil phagocytosis by IL-15, as demonstrated by functional studies Related references: PMID:15123770
• The protein is localized at the cell membrane and cell surface, consistent with its role as a transmembrane receptor subunit.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate receptor stability and signaling.
• Alternative splicing of IL2RG produces multiple isoforms, potentially modulating receptor function and tissue-specific expression.
• Mutations in IL2RG are associated with X-linked severe combined immunodeficiency (SCID), highlighting its critical role in immune system development.
• The predicted molecular weight of the full-length protein is approximately 42 kDa, consistent with the observed 42 kDa band in Western blotting applications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250-350, which lies within the intracellular domain of human CD132. This domain is relatively conserved, supporting cross-reactivity with mouse, but verification in your specific experimental system is recommended.
• For Western blot analysis, a primary antibody dilution should be optimized; a band at approximately 42 kDa is expected in lysates from IL-2RG-expressing cells.
• For ELISA applications, the antibody may be suitable for both direct and sandwich formats. Pair with a detection antibody recognizing a non-overlapping epitope for best results.
• Given the biological role of CD132, appropriate positive control samples include lymphoid cell lines or immune tissues, while knockout or knockdown models can serve as negative controls.

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CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials and technical services. For projects targeting immune signaling pathways, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Whether scaling up production or sourcing rare-specificity antibodies like anti-CD132, our team bridges the gap from concept to clinic.