Anti-CD132/IL-2 R gamma Polyclonal Antibody for WB and ELISA - P31785

Core Product Specifications and Parameters

Biological Background: CD132/IL-2 R gamma Function and Localization

• CD132/IL-2 R gamma, also known as the common gamma chain (γc), interleukin-2 receptor subunit gamma (IL-2RG), or p64, is encoded by the IL2RG gene on human chromosome Xq13.1.
• It serves as a common subunit for receptors of multiple interleukins, including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, playing a central role in immune cell development and function.
• In association with IL-15 receptor alpha (IL15RA), it participates in the stimulation of neutrophil phagocytosis by IL-15. Related references: PMID:15123770
• The protein is a single-pass type I membrane receptor, localized to the cell membrane and cell surface, where it forms functional receptor complexes with ligand-specific alpha and beta subunits.
• Post-translational modifications include glycosylation and phosphorylation, as annotated by UniProt keywords, which are critical for receptor stability and signaling.
• Mutations in IL2RG cause X-linked severe combined immunodeficiency (XSCID), characterized by profound defects in T, B, and NK cell development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 290-369 of human CD132/IL-2 R gamma, targeting the cytoplasmic domain; this region is relatively conserved, which may explain cross-reactivity with mouse. Users should verify reactivity in their specific experimental systems.
• This polyclonal antibody is applicable for Western blot (WB) and ELISA. For WB, a band around 42 kDa is expected, but consider that glycosylation may cause a slightly higher molecular weight.
• Given the cytokine receptor's role in immune signaling, appropriate positive controls include human or mouse lymphoid cells (e.g., T cells, NK cells) expressing IL2RG.
• To avoid non-specific binding in ELISA, consider optimizing coating antigen and antibody concentration using recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research institutes, and clinical labs a streamlined single-vendor solution for IVD raw materials, from concept to commercialization. This anti-CD132/IL-2 R gamma polyclonal antibody is part of our extensive portfolio of validated immunoassay reagents supporting immune function and cytokine research. Our team can also source rare targets, provide matched antibody pairs, and supply bulk ancillary materials to accelerate your diagnostic development.