Anti-CD132 Rabbit Polyclonal Antibody for WB, ELISA - P34902

Core Product Specifications and Parameters

Biological Background: CD132 Function and Localization

• CD132 (also known as Interleukin-2 receptor subunit gamma, IL-2RG, gammaC, p64) serves as the common subunit for the receptors of multiple interleukins, playing a central role in cytokine signaling and immune regulation. Related references: PMID:7718508
• In association with IL15RA, CD132 is likely involved in IL-15-dependent stimulation of neutrophil phagocytosis (By similarity).
• CD132 is a single-pass type I membrane glycoprotein located on the cell surface and plasma membrane.
• The protein features an N-terminal signal peptide, an extracellular region with conserved disulfide bonds, a transmembrane helix, and a cytoplasmic tail required for signal transduction.
• It undergoes N-linked glycosylation, which modulates receptor stability and ligand-binding affinity.
• The three-dimensional structure of CD132 has been resolved, aiding in the understanding of cytokine receptor assembly and activation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 23–263 of mouse CD132, corresponding to the extracellular domain; thus, the antibody is likely to recognize the native receptor on cell surfaces and may be suitable for ELISA applications requiring conformational epitopes.
• For Western blot, anticipate a band between 42 and 55 kDa due to glycosylation; validate detection in relevant mouse tissue or cell lysates and optimize antibody dilution and blocking conditions.
• Cross-reactivity with rat CD132 suggests potential use in rat samples; however, confirm reactivity by testing in rat-derived lysates or ELISA.
• For ELISA, consider pairing with a capture antibody targeting a distinct epitope if developing a sandwich assay; perform checkerboard titration for optimal performance.

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