Anti-CD130/gp130 Rabbit Monoclonal Antibody for Flow Cytometry - Q00560

Core Product Specifications and Parameters

Biological Background: CD130/gp130 Function and Localization

• Interleukin-6 receptor subunit beta (IL6ST), also known as gp130 or CD130, is a shared signal-transducing subunit for multiple cytokine receptors, including those for IL-6, LIF, OSM, CNTF, IL-11, CTF1, CLCF1, and MT-RNR2/humanin. Related references: PMID:1602143

• Upon ligand binding, gp130 homodimerizes (e.g., with IL-6 or IL-11) or heterodimerizes with LIFR or OSMR, activating associated JAK kinases and downstream STAT transcription factors. Related references: PMID:10661409, PMID:1602143

• The IL-6/gp130 pathway induces negative regulators SOCS1 and SOCS3, which terminate signaling by inhibiting JAK activity. Related references: PMID:9202125

• gp130 also activates YAP and NOTCH pathways independently of STAT3 to control inflammation-induced epithelial regeneration. Related references: PMID:25731159

• This receptor is essential for embryonic development, immune regulation, hematopoiesis, pain control, bone metabolism, and survival of motor/sensory neurons. Related references: PMID:10661409, PMID:25057188, PMID:26255596, PMID:10377352

• Subcellularly, gp130 localizes to the cell membrane. It is broadly expressed in many tissues, including brain, heart, thymus, spleen, kidney, lung, and liver.

• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, consistent with its role as a transmembrane signaling receptor.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 23–617) covers the entire extracellular domain of mouse gp130; the antibody likely recognizes native surface gp130, making it suitable for flow cytometry.
• For flow cytometry, use appropriate isotype controls and titrate the antibody to determine optimal staining concentration.
• Verify performance in relevant mouse cell lines or primary cells known to express gp130 (e.g., immune cells, hepatocytes).
• Consider that cytokine stimulation may modulate gp130 surface levels; thus, staining conditions should be carefully controlled.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories. This high-quality rabbit monoclonal antibody against CD130/gp130 supports cytokine signaling research and flow cytometry applications. From validated antibody pairs and bulk ancillary reagents to rare target sourcing, our integrated supply chain ensures the reliable delivery of critical components to accelerate your diagnostic development.