Anti-CD127/IL7R Rabbit Polyclonal Antibody for WB, ELISA - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL7R Function and Localization

• The IL7R gene encodes the interleukin-7 receptor alpha chain, also known as CD127 or CDw127.
• CD127 functions as the receptor for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP), playing a critical role in lymphocyte development and homeostasis.
• The receptor is a type I membrane protein located primarily on the cell membrane, but alternative splicing generates secreted isoforms.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate receptor signaling.
• Mutations in IL7R are associated with severe combined immunodeficiency (SCID) and other immunological disorders.
• The canonical isoform has a predicted molecular weight of 51.6 kDa, aligning with the observed 52 kDa band.

Experimental Guidance and Technical Tips

• For Western blot, consider using human or mouse lymphoid cell lines as positive controls, given the reported cross-reactivity.
• Optimize blocking and antibody dilution for each application; typical starting concentrations may need empirical determination.
• The immunogen corresponds to the N-terminal extracellular region (aa 21-239), suggesting the antibody likely recognizes extracellular epitopes; validate in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material sourcing partner, CamelBio offers this high-quality polyclonal antibody and can support your diagnostic development with validated antibody pairs, optimized clones, and bulk ancillary reagents. Our team specializes in rapid procurement of rare-target antibodies and custom production to meet your unique project needs in immunological research and assay development.