Anti-CD127/IL7R Monoclonal Antibody for WB, IF-P, ELISA - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL7R Function and Localization

• CD127 (IL7R) is a receptor for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP).
• Encoded by the IL7R gene, this protein is a type I membrane glycoprotein belonging to the cytokine receptor family.
• Alternative splicing generates multiple isoforms, including a secreted form (sIL-7R).
• It localizes to the cell membrane and can also be secreted into the extracellular space.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination, as well as disulfide bond formation.
• Mutations in IL7R are linked to autosomal recessive severe combined immunodeficiency (SCID), underlining its importance in immune function.

Experimental Guidance and Technical Tips

• The immunogen maps to the cytoplasmic domain (aa 300-400); permeabilization may be necessary for intracellular detection in immunofluorescence.
• For Western blot, the expected band is near 52 kDa; glycosylation could shift the apparent molecular weight.
• In ELISA applications, consider pairing with an extracellular domain-targeting antibody to avoid epitope competition.
• Sequence alignment of the immunogen region across human, mouse, and rat is recommended to confirm cross-reactivity.
• Soluble CD127 (sCD127) may be detected in culture supernatants or serum if the epitope remains accessible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic partner for diagnostic manufacturers and laboratories, offering reliable access to validated raw materials. This Anti-CD127/IL7R monoclonal antibody is part of a broad portfolio that includes matched antibody pairs, ancillary reagents, and custom bulk supply options. Our expert support helps streamline assay development for immunology and cytokine receptor research, ensuring consistent quality from concept to clinic.