Anti-CD127/IL-7Rα Monoclonal Antibody () for Flow Cytometry - P16871

Core Product Specifications and Parameters

Biological Background: IL-7Rα Function and Localization

• The interleukin-7 receptor subunit alpha (IL-7Rα, also known as CD127 or CDw127) is encoded by the IL7R gene and functions as a receptor for interleukin-7 (IL-7).
• IL-7Rα also serves as a receptor for thymic stromal lymphopoietin (TSLP), implicating it in both lymphoid development and inflammatory processes.
• Alternative splicing of IL7R transcripts generates both membrane-bound and secreted isoforms, with the soluble form (sIL-7R) modulating IL-7 bioavailability.
• Subcellularly, IL-7Rα localizes to the cell membrane, while the soluble isoform is secreted into the extracellular space.
• The protein undergoes post-translational modifications typical of cell surface receptors, including glycosylation, phosphorylation, and ubiquitination.
• Mutations in IL7R are linked to severe combined immunodeficiency (SCID), highlighting its essential role in immune cell development.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is ready-to-use for flow cytometry without secondary detection.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Validate the antibody’s reactivity in your model system using human or cynomolgus monkey samples, as cross-reactivity has been confirmed with these species.
• The immunogen corresponds to the extracellular domain (amino acids 21–236), ensuring recognition of surface-expressed IL-7Rα.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, offering a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. For immunology and inflammation research, we provide monoclonal antibodies against key cytokine receptors like IL-7Rα, along with bulk supply and custom development services. Our end-to-end support helps diagnostic manufacturers and labs accelerate assay development and biomarker discovery.