Anti-CD123/IL-3R Alpha Polyclonal Antibody (Rabbit) for WB and ELISA - P26951

Core Product Specifications and Parameters

Biological Background: Interleukin-3 receptor subunit alpha Function and Localization

• CD123, encoded by the IL3RA gene, is the ligand-binding alpha subunit of the interleukin-3 receptor, also known as IL-3R-alpha.
• It mediates IL-3-dependent signaling essential for hematopoietic stem cell proliferation and lineage differentiation, primarily through JAK2/STAT5 and PI3K/AKT pathways.
• Ligand engagement triggers heterodimerization with the common beta chain (IL3RB/CSF2RB), activating downstream kinases (PubMed:10527461). Related references: PMID:10527461
• CD123 is predominantly expressed on hematopoietic progenitor cells, monocytes, and B-lymphocytes, with elevated levels in certain leukemic blasts, making it a diagnostic marker and therapeutic target in AML.
• The receptor is a type I transmembrane protein located on the plasma membrane, with an extracellular domain containing a cytokine receptor homology module.
• Post-translational modifications include N-linked glycosylation and disulfide bonds critical for structural integrity, as well as ubiquitination that may influence receptor downregulation.
• Alternative splicing yields multiple transcript variants, potentially producing isoforms with truncated cytoplasmic tails or altered ligand affinity.

Experimental Guidance and Technical Tips

• The immunogen spanning amino acids 20–305 covers the extracellular domain; the antibody is likely to recognize native, denatured, and possibly soluble CD123 forms.
• For WB, a specific band around 43 kDa is expected, but glycosylation may shift the apparent mass higher (commonly 55–70 kDa); deglycosylation controls are recommended.
• In ELISA, this polyclonal antibody can be used for antigen capture or detection; optimal dilution should be determined empirically in the user’s assay system.
• Reported mouse cross-reactivity enables comparative studies, but validation by parallel staining or blotting of mouse cells is advised.
• As a polyclonal preparation, lot-to-lot consistency may vary; include a positive control (e.g., TF-1 or OCI-AML3 cell lysates) and titrate new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, providing diagnostic developers with validated antibodies, antigens, and ancillary reagents. For CD123-related assay development—particularly in hematologic malignancy diagnostics—CamelBio offers optimized polyclonal antibodies and sourcing services for rare targets. Our technical support and supply chain management streamline your project from research to regulatory approval.