Anti-CD123 Rabbit Monoclonal Antibody for FC, ELISA - P26952

Core Product Specifications and Parameters

Biological Background: CD123 Function and Localization

• Interleukin-3 receptor subunit alpha (CD123, also known as IL3RA, SUT-1) is the alpha chain of the IL-3 receptor, a cell surface receptor expressed on hematopoietic progenitor cells, monocytes, and B-lymphocytes, where it regulates hematopoietic cell production and differentiation.
• Upon IL-3 binding, CD123 heterodimerizes with the common beta chain (IL3RB/CSF2RB) to activate signaling pathways including JAK2 and PI3K, driving cell proliferation and differentiation.
  Related references: PMID:10477686, PMID:31990690
• Activated JAK2 phosphorylates STAT5, initiating a STAT5-mediated transcriptional program essential for lineage-restricted cell differentiation.
  Related references: PMID:10376805
• The receptor localizes to the cell membrane and endomembrane system, consistent with its function as a transmembrane signaling protein.
• CD123 undergoes alternative splicing and post-translational modifications including glycosylation, disulfide bond formation, isopeptide bonding, and ubiquitination, which may modulate its expression and signaling.
• The predicted molecular weight of the unmodified receptor is approximately 43 kDa, featuring an N-terminal signal peptide and a single transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of mouse CD123 (residues 17-331), which is predicted to generate antibodies that recognize the native surface receptor; this design supports applications such as flow cytometry (FC) and ELISA.
• For flow cytometry, use an appropriate fluorophore-conjugated secondary antibody and validate staining in mouse hematopoietic cell lines or primary cells known to express CD123, such as bone marrow-derived progenitor cells.
• For ELISA, consider using this antibody as either a capture or detection reagent; pairing with a matched detection antibody may improve sensitivity. Optimization of coating antigen concentration and antibody titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing high-quality reagents like this CD123 monoclonal antibody to support hematopoiesis, cytokine signaling, and cancer research. Beyond monoclonal antibodies, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.