Anti-CD11c Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P20702

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X (CD11c) Function and Localization

• Integrin alpha-X (CD11c) is a member of the integrin family and the alpha chain of the leukocyte adhesion receptor p150,95 (integrin alpha-X/beta-2). It is also known as CD11 antigen-like family member C, Leu M5, and leukocyte adhesion glycoprotein p150,95 alpha chain.
• Integrin alpha-X/beta-2 serves as a receptor for fibrinogen, recognizing the G-P-R sequence, and mediates cell-cell interactions during inflammatory responses, particularly crucial for monocyte adhesion and chemotaxis.
• It also functions as a receptor for the erythrocyte-specific adhesion molecule ICAM4, mediating erythrophagocytosis. Related references: PMID:16985175
• CD11c is predominantly expressed in monocytes and granulocytes, consistent with its role in leukocyte adhesion processes.
• The protein localizes to the cell membrane as a single-pass type I membrane protein, with a large extracellular domain involved in ligand binding.
• Post-translational modifications include disulfide bonds and glycosylation, classifying it as a glycoprotein essential for structural stability and function.
• The integrin alpha-X chain contains calcium and magnesium binding sites, which are critical for integrin activation and ligand interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the extracellular domain (amino acids 311–410), which may enhance specificity for native CD11c detection in various assays.
• For Western blot (WB), expect a band near 128 kDa; consider reducing conditions to resolve the integrin heterodimer if necessary.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate antigen retrieval methods and include positive control tissues such as spleen or lymph node, which are rich in monocytes.
• For ELISA, pair with a suitable detection antibody and optimize coating conditions to capture soluble or lysate-derived CD11c.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For integrin alpha-X (CD11c) research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to accelerate your diagnostic development in inflammatory and hematopoietic disease areas.