Anti-CD11b Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11215

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• CD11b (integrin alpha-M), encoded by the ITGAM gene, is the alpha chain of the MAC-1 integrin complex, also known as CR-3, MO1, and CD11B (PubMed:1346576).
• ITGAM/ITGB2 integrin mediates diverse adhesive interactions in monocytes, macrophages, and granulocytes, including uptake of complement-coated particles via recognition of the iC3b fragment. Related references: PMID:20008295, PMID:9558116
• This integrin serves as a receptor for fibrinogen (recognizing P1 and P2 peptides of the gamma chain) and factor X, facilitating immune cell adhesion and migration.
• It regulates neutrophil migration and, in association with ITGB2, is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils. Related references: PMID:28807980, PMID:21193407
• As a receptor for ICAM1, it mediates adhesion between stimulated neutrophils and endothelial cells, a key step in inflammation. Related references: PMID:1980124
• Subcellular localization is primarily at the cell membrane and membrane rafts, consistent with its role in signal transduction.
• Predominantly expressed in monocytes and granulocytes, including neutrophils, supporting its central function in innate immunity.
• The protein undergoes alternative splicing and post-translational modifications such as glycosylation and disulfide bond formation, which are critical for its structural integrity and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal sequence (aa 1-100) of human CD11b, which may confer high specificity for the full-length protein.
• For Western Blot, a band at approximately 127 kDa is expected; consider using reducing conditions and validate with positive control lysates from human monocytes or neutrophils.
• In immunohistochemistry (IHC-P), antigen retrieval and blocking steps may be optimized to reduce background in formalin-fixed paraffin-embedded tissues.
• For ELISA, the antibody can be used as a capture or detection reagent; ensure proper pairing validation for sandwich immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD development by providing validated antibody raw materials like this CD11b monoclonal antibody, essential for research into inflammatory and immune-mediated diseases. Beyond individual antibodies, our portfolio includes optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your project from concept to clinic. Rely on CamelBio as your dedicated partner to streamline the sourcing of high-quality raw materials for diagnostic assays.