Anti-CD11b (ITGAM) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05555

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• Integrin alpha-M (CD11b), encoded by the Itgam gene, is a member of the integrin alpha chain family and combines with the beta-2 subunit (ITGB2/CD18) to form the complement receptor CR3 (Mac-1). Alternative splicing yields multiple isoforms.
• The ITGAM/ITGB2 integrin mediates adhesive interactions of monocytes, macrophages, and granulocytes, including binding to the iC3b fragment of complement C3 and facilitating uptake of complement-coated particles and pathogens (By similarity).
• Beyond complement, CD11b serves as a receptor for fibrinogen, factor X, and ICAM1; it recognizes RGD motifs and the fibrinogen gamma chain P1/P2 peptides, thereby promoting leukocyte adhesion and migration.
• The receptor plays a central role in neutrophil migration and, together with CD18, is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils (By similarity).
• It may regulate phagocytosis-induced apoptosis in extravasated neutrophils, contributing to the resolution of inflammation (By similarity).
• In the brain, CD11b is required together with TYROBP/DAP12 in microglia for production of superoxide ions that drive developmental neuronal apoptosis. Related references: PMID:18685038
• Subcellularly, CD11b is localized at the cell membrane and is enriched in membrane rafts.
• Tissue-specific expression is predominantly observed in monocytes, granulocytes, a subset of peritoneal mast cells, and microglia.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 143–337 of mouse CD11b, covering a portion of the extracellular N-terminal region. This design may allow recognition of both native and denatured protein conformations, making it suitable for Western blot, immunohistochemistry, and ELISA.
• For Western blot analysis, CD11b migrates at approximately 127 kDa. Consider using a 6–12% SDS-PAGE gel and appropriate positive controls (e.g., macrophage or granulocyte lysates) to verify specificity.
• Due to the predicted glycosylation and disulfide-bonded structure, reducing conditions and optimized sample preparation may influence detection. Validate the antibody in the relevant sample system to confirm performance.
• This antibody is reported to cross-react with human and rat CD11b; however, sequence differences may affect affinity in these species, so independent validation in the target species is recommended.
• For IHC-P applications, antigen retrieval protocols and tissue fixation methods should be optimized for the specific sample type to achieve the best signal-to-noise ratio.
• In ELISA, the antibody can be used for capture or detection of CD11b; consider pairing it with a compatible monoclonal antibody in a sandwich ELISA format to enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with a one-stop supply chain for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CD11b polyclonal antibody is part of our extensive portfolio for immunology, inflammation, and infectious disease research applications. For customized sourcing of rare targets or scaling up to industrial quantities, our team offers end-to-end support from concept to clinic—ensuring you have the critical reagents needed to accelerate your diagnostic development.