Anti-CD11a/LFA-1A/ITGAL Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P20701

Core Product Specifications and Parameters

Biological Background: Integrin alpha-L (CD11a/LFA-1) Function and Localization

• Integrin alpha-L (ITGAL, CD11a) pairs with integrin beta-2 (ITGB2) to form lymphocyte function-associated antigen 1 (LFA-1), a key heterodimeric adhesion receptor belonging to the integrin family.
• LFA-1 serves as a receptor for multiple intercellular adhesion molecules (ICAM1, ICAM2, ICAM3, ICAM4), junctional adhesion molecule F11R, and the ubiquitin-like protein ISG15, mediating leukocyte adhesion and signaling. Related references: PMID:10846180, PMID:12526797, PMID:1676048, PMID:11812992, PMID:15528364, PMID:29100055
• The receptor is essential for leukocyte-endothelial cell interactions, T-cell migration, cytotoxic T-cell-mediated killing, and antibody-dependent cellular cytotoxicity by granulocytes and monocytes. Related references: PMID:11812992, PMID:15356110
• At the immunological synapse, LFA-1 translates T-cell receptor engagement and ICAM1 ligand density into graded adhesion, and it also contributes to apoptotic neutrophil phagocytosis and neuron-leukocyte adhesion via ICAM5. Related references: PMID:38195629, PMID:23775590, PMID:10741396
• ITGAL is primarily expressed on the surface of leukocytes and localizes to the plasma membrane and membrane rafts, consistent with its adhesion and signaling roles.
• The protein is a glycoprotein that binds calcium and magnesium, with disulfide bonds and phosphorylation regulating its structure and function.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 26–340, corresponding to the extracellular region of human ITGAL, which is conserved in mouse and rat, supporting the observed cross-reactivity. Validate in the relevant sample system for your target species.
• For Western blot (WB), the predicted molecular weight of unglycosylated human ITGAL is ~129 kDa, but glycosylation may cause a higher apparent mass. Use leukocyte-rich lysates (e.g., Jurkat, THP-1) as positive controls.
• For immunofluorescence (IF/ICC, IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization conditions, as the target is a transmembrane protein. Antigen retrieval may be required for paraffin-embedded tissues.
• As a polyclonal antibody, batch-to-batch variability can occur; consider screening new lots or using a monoclonal pairing antibody if reproducibility is critical for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories worldwide a streamlined one-stop platform for IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. This anti-CD11a/LFA-1A polyclonal antibody supports leukocyte adhesion, immune function, and inflammation research, and we can help you source complementary reagents or rare targets to accelerate your development. Let us bridge your project from concept to clinic with reliable supply solutions.