Anti-CD11a/LFA-1A/ITGAL Rabbit Polyclonal Antibody for WB, ELISA - P20701

Core Product Specifications and Parameters

Biological Background: CD11a/LFA-1A/ITGAL Function and Localization

• Integrin alpha-L (ITGAL/CD11a/LFA-1A) is a leukocyte-specific integrin alpha chain that pairs with integrin beta-2 (ITGB2) to form the LFA-1 complex. It is predominantly expressed on leukocytes and localizes to the cell membrane and membrane rafts.
• Functions as a receptor for intercellular adhesion molecules ICAM1, ICAM2, ICAM3, and ICAM4, mediating leukocyte adhesion and transmigration across endothelia. Related references: PMID:10846180 PMID:12526797 PMID:1676048
• Engages the junctional adhesion molecule F11R (JAM-1) to facilitate leukocyte adhesion and transmigration. Related references: PMID:11812992 PMID:15528364
• Serves as a receptor for the ubiquitin-like protein ISG15, enhancing immune signaling and antiviral responses. Related references: PMID:29100055
• Contributes to natural killer cell cytotoxicity. Related references: PMID:15356110
• At the immunological synapse, integrates TCR engagement and ICAM1 density to modulate T-cell adhesion. Related references: PMID:38195629
• Cooperates with ICAM3 to mediate apoptotic neutrophil phagocytosis by macrophages. Related references: PMID:23775590
• Functions as a receptor for neuron-specific ICAM5, enabling neuron-leukocyte adhesion. Related references: PMID:10741396

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 26–306), which may allow detection of both full-length and potential splice variants if epitopes are conserved.
• For Western blotting, expect a band near 129 kDa. Use reduced denaturing conditions, and validate in human leukocyte lysates to confirm specificity.
• Cross-reactivity with rat is indicated; include appropriate species-specific controls when analyzing rat samples.
• For ELISA, optimize antibody titration and assay conditions based on the target antigen format (recombinant protein or cell-based).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibodies, antigen recombinant proteins, and ancillary reagents to accelerate immunology and diagnostic research. This CD11a/LFA-1A/ITGAL polyclonal antibody exemplifies our capacity to supply high-quality reagents for leukocyte adhesion and immune cell interaction studies. Explore our portfolio for additional targets in cell adhesion, inflammation, and immune-oncology.