Anti-CD11a Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P20701

Core Product Specifications and Parameters

Biological Background: CD11a Function and Localization

• Functions as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4, mediating leukocyte adhesion and immune interactions.
  Related references:
  PMID:10846180
  PMID:12526797
  PMID:1676048

• Serves as a receptor for F11R and the secreted form of ISG15; the ISG15 interaction is mediated specifically by ITGAL.
  Related references:
  PMID:11812992
  PMID:15528364
  PMID:29100055

• Contributes to T-cell mediated killing, antibody-dependent cytotoxicity, and natural killer cell cytotoxicity.
  Related references:
  PMID:15356110

• Involved in leukocyte transmigration and the formation of the immunological synapse, translating TCR engagement and ICAM1 density into graded adhesion.
  Related references:
  PMID:11812992
  PMID:38195629

• Mediates apoptotic neutrophil phagocytosis by macrophages in association with ICAM3.
  Related references:
  PMID:23775590

• Functions as a receptor for neuron-specific ICAM5, facilitating neuron-leukocyte adhesion.
  Related references:
  PMID:10741396

• Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (by similarity).

• Localizes to the cell membrane and membrane rafts; expression is restricted to leukocytes. Undergoes alternative splicing and post-translational modifications including phosphorylation, glycosylation, and disulfide bond formation; contains calcium and magnesium binding sites.

Experimental Guidance and Technical Tips

• For Western blotting, the protein migrates at 119–134 kDa due to isoforms and glycosylation; reducing conditions and appropriate molecular weight markers are recommended.
• In IHC-P, antigen retrieval protocols may be necessary; validate in leukocyte-rich tissues such as lymph node or spleen.
• The antibody is suitable for ELISA applications; consider pairing with a detection antibody for sandwich ELISA formats.
• Cross-reactivity with mouse and rat is reported; validate in the relevant sample system with appropriate controls to confirm specificity.
• As a monoclonal antibody, it offers batch-to-batch consistency; however, confirm target specificity using knockout lysates or blocking peptides where available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focused on immunology and leukocyte biology, this anti-CD11a antibody facilitates streamlined procurement for leukocyte adhesion and inflammation studies. CamelBio also specializes in sourcing rare-target raw materials, ensuring you have access to the critical reagents needed from concept to clinic.