Anti-CD11a Rabbit Monoclonal Antibody for WB, FC, ELISA - P24063

Core Product Specifications and Parameters

Biological Background: CD11a Function and Localization

• Integrin alpha-L (CD11a) is a ~128 kDa transmembrane glycoprotein that pairs with integrin beta-2 (CD18) to form the LFA-1 heterodimer, a key mediator of leukocyte adhesion and immune cell interactions.
• LFA-1 serves as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4, driving leukocyte-endothelial adhesion, T-cell migration, and natural killer cell cytotoxicity. Related references: PMID:2051027, PMID:16234355, PMID:24158516
• Beyond ICAM binding, ITGAL recognizes the secreted ubiquitin-like protein ISG15 and junctional adhesion molecule F11R, expanding its functional repertoire. Related references: PMID:29100055
• At the immunological synapse, LFA-1 acts as a graded adhesion platform, translating TCR engagement and ICAM1 density into mechanical stability and signaling output (By similarity).
• Subcellularly, ITGAL localizes to the plasma membrane and membrane rafts; its expression is restricted to leukocytes, consistent with its role in immune surveillance.
• The protein is subject to N-glycosylation, phosphorylation, and disulfide bond formation, and requires divalent cations (calcium, magnesium) for ligand binding and activation.
• LFA-1 is essential for lymphopoiesis, contributing to the generation of common lymphoid progenitor cells in bone marrow. Related references: PMID:25108025
• In addition, LFA-1 cooperates with ICAM3 to mediate macrophage phagocytosis of apoptotic neutrophils, aiding resolution of inflammation (By similarity).

Experimental Guidance and Technical Tips

• For Western blotting, a predominant band near 128 kDa is expected under reducing conditions; inclusion of a positive control (e.g., mouse spleen or lymph node lysate) and validation in knockout/cell-line models is advisable.
• In flow cytometry, use viable, non-fixed cells to preserve surface epitope integrity; a fluorophore-conjugated secondary antibody or direct labeling is recommended for optimal detection.
• For ELISA applications, consider using recombinant mouse CD11a protein as a standard and titrating the antibody to determine optimal coating and detection concentrations.
• The immunogen (residues 149–340) lies within the extracellular head domain; this region is conserved between mouse and rat, supporting the reported cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material provider, offering one-stop access to validated antibody pairs, optimized monoclonal antibodies like this CD11a clone, and bulk ancillary reagents for immunoassay development. Supporting leukocyte adhesion and immune function research, we help diagnostic manufacturers streamline procurement from discovery to clinical application. For rare‑target sourcing and custom antibody generation, our technical consulting services are available to meet your development needs.