Anti-CD119 Polyclonal Antibody for WB, ELISA - P15261

Core Product Specifications and Parameters

Biological Background: CD119 Function and Localization

• CD119, also known as Interferon gamma receptor 1 (IFNGR1) or IFN-gamma-R-alpha, is a single-pass type I membrane protein that serves as the ligand-binding subunit of the interferon gamma receptor.
• The receptor plays a crucial role in mediating antimicrobial, antiviral, and antitumor immune responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:20926559, PMID:27286456
• CD119 associates with the accessory factor IFNGR2 to form a functional receptor complex capable of signal transduction. Related references: PMID:2137461, PMID:2530216, PMID:2530582
• Upon interferon gamma binding, the intracellular domain of CD119 undergoes a conformational change that permits the recruitment and activation of JAK1 and JAK2 kinases; activated JAK1 then phosphorylates CD119, creating a docking site for STAT1. Related references: PMID:19889125
• Phosphorylated STAT1 dimerizes and translocates to the nucleus to stimulate transcription of interferon-gamma-responsive genes. STAT3 can also be activated via this pathway, although to a lesser extent. Related references: PMID:15284232
• The phosphorylated intracellular domain also provides a binding site for SOCS1, which negatively regulates JAK-STAT signaling by competing with STAT1 for receptor docking. Related references: PMID:15522878
• CD119 is localized primarily at the cell membrane and contains structural features including an immunoglobulin-like domain, disulfide bonds, glycosylation sites, and a transmembrane helix; it is also subject to phosphorylation and ubiquitin conjugation.
• The mature protein has a calculated molecular weight of approximately 52.3 kDa, consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• For Western blotting, use appropriate protein extraction and reducing conditions; the expected band is ~52 kDa. Perform a dilution series to determine the optimal primary antibody concentration for your specific sample type.
• For ELISA applications, consider using the antibody as a capture or detection reagent; a checkerboard titration with recombinant mouse CD119 or cell lysates is recommended to establish working concentrations.
• This antibody has been validated for use with mouse samples; cross-reactivity with other species has not been tested and should be verified experimentally if needed.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed in pilot experiments when switching to a new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable antibodies and technical support from concept to clinic. We offer validated polyclonal antibodies like this anti-CD119 reagent, as well as monoclonal antibodies, recombinant proteins, and ancillary reagents covering immune response and receptor signaling targets. In addition to catalog products, CamelBio’s sourcing network can assist with bulk orders, custom immunogen design, and rare target procurement to accelerate your assay development.